Literature summary for 1.1.1.213 extracted from

Nakamura, S.; Oda, M.; Kataoka, S.; Ueda, S.; Uchiyama, S.; Yoshida, T.; Kobayashi, Y.; Ohkubo, T.
Apo- and holo-structures of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831. Loop-helix transition induced by coenzyme binding (2006), J. Biol. Chem., 281, 31876-31884.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme with NADH, hanging drop vapour diffusion method, 4°C, in presence of 1.4 M ammonium sulfate, 0.14 M NaCl, and 0.1 M Tris, pH 9.0, rod cluster crystals appear within 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	2 * 26000	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5alpha-dihydrotestosterone + NAD(P)H + H+	Pseudomonas sp.	-	5alpha-androstane-3alpha,17beta-diol + NAD(P)+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5alpha-dihydrotestosterone + NAD(P)H + H+	-	Pseudomonas sp.	5alpha-androstane-3alpha,17beta-diol + NAD(P)+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 26000	Pseudomonas sp.
More	comparison of the folding topologies found in the crystal structures of the holo-enzyme and the apo-enzyme, structural changes in solution, overview	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
3alpha-hydroxysteroid dehydrogenase	-	Pseudomonas sp.
3alphaHSD	-	Pseudomonas sp.
More	cf. EC 1.1.1.50, the enzyme belongs to the AKR1C family	Pseudomonas sp.

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)H	cofactor binding structure, overview	Pseudomonas sp.

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Release 2023.1 (January 2023)