Inhibitors | Comment | Organism | Structure |
---|---|---|---|
indomethacin | non-steroidal anti-inflamatory drug, uncompetitive against NAD+, competitive against androsterone | Rattus norvegicus | |
non-steroidal anti-inflamatory drug | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
5alpha-androstan-3,17-dione | - |
Rattus norvegicus | |
0.033 | - |
NADH | - |
Rattus norvegicus | |
0.047 | - |
3alpha-hydroxy-5alpha-androstan-17-one | - |
Rattus norvegicus | |
0.76 | - |
NAD+ | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100 | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | binding and release or pyridine is rate-limiting | Rattus norvegicus | |
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | bi bi mechanism, pyridine nucleotide binds first and leaves last | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.93 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NADPH | - |
Rattus norvegicus | 3alpha,17beta-dihydroxy-5alpha-androstan + NADP+ | - |
r | |
3alpha-hydroxy-5alpha-androstan-17-one + NAD+ | - |
Rattus norvegicus | 5alpha-androstan-3,17-dione + NADH | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | A-specific | Rattus norvegicus | |
NADH | A-specific, hydride transfer occurs from the 4-pro-R-position of the cofactor to the C-3 position of the steroid | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00057 | - |
indomethacin | - |
Rattus norvegicus |