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Literature summary for 1.1.1.213 extracted from

  • Askonas, L.J.; Ricigliano, J.W.; Penning, T.M.
    The kinetic mechanism catalysed by homogeneous rat liver 3alpha-hydroxysteroid dehydrogenase. Evidence for binary and ternary dead-end complexes containing non-steroidal anti-inflammatory drugs (1991), Biochem. J., 278, 835-841.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
indomethacin non-steroidal anti-inflamatory drug, uncompetitive against NAD+, competitive against androsterone Rattus norvegicus
non-steroidal anti-inflamatory drug
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
5alpha-androstan-3,17-dione
-
Rattus norvegicus
0.033
-
NADH
-
Rattus norvegicus
0.047
-
3alpha-hydroxy-5alpha-androstan-17-one
-
Rattus norvegicus
0.76
-
NAD+
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100 Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ binding and release or pyridine is rate-limiting Rattus norvegicus
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ bi bi mechanism, pyridine nucleotide binds first and leaves last Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.93
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
Rattus norvegicus 3alpha,17beta-dihydroxy-5alpha-androstan + NADP+
-
r
3alpha-hydroxy-5alpha-androstan-17-one + NAD+
-
Rattus norvegicus 5alpha-androstan-3,17-dione + NADH
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+ A-specific Rattus norvegicus
NADH A-specific, hydride transfer occurs from the 4-pro-R-position of the cofactor to the C-3 position of the steroid Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00057
-
indomethacin
-
Rattus norvegicus