Cloned (Comment) | Organism |
---|---|
expressed as a recombinant His-tag fusion protein in Escherichia coli BL21(DE3) cells | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
DL-glyceraldehyde | in 50 mM sodium phosphate at pH 7.0 and 30°C | Zea mays | |
108.1 | - |
D-ribose | in 50 mM sodium phosphate at pH 7.0 and 30°C | Zea mays | |
179.5 | - |
D-xylitol | in 50 mM sodium phosphate at pH 7.0 and 30°C | Zea mays | |
399.5 | - |
D-sorbitol | in 50 mM sodium phosphate at pH 7.0 and 30°C | Zea mays | |
448.4 | - |
D-arabinose | in 50 mM sodium phosphate at pH 7.0 and 30°C | Zea mays | |
466.8 | - |
D-xylose | in 50 mM sodium phosphate at pH 7.0 and 30°C | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34560 | - |
calculated from amino acid sequence | Zea mays |
34560 | - |
recombinant enzyme, MALDI-TOF mass spectrometry | Zea mays |
34561 | - |
1 * 34561, MALDI-TOF mass spectrometry | Zea mays |
35000 | - |
SDS-PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | A2T1W7 | - |
- |
Purification (Comment) | Organism |
---|---|
agarose-IDA-Ni resin column chromatography, gel filtration | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
seed | - |
Zea mays | - |
Storage Stability | Organism |
---|---|
-20°C, the activity of the recombinant maize AKR4C7 enzyme is stable with no significant change in activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADPH + H+ | - |
Zea mays | arabitol + NADP+ | - |
? | |
D-glucose + NADPH + H+ | low affinity for D-glucose | Zea mays | D-sorbitol + NADP+ | - |
? | |
D-ribose + NADPH + H+ | - |
Zea mays | D-ribitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Zea mays | D-xylitol + NADP+ | - |
? | |
DL-glyceraldehyde + NADPH + H+ | - |
Zea mays | glycerol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34561, MALDI-TOF mass spectrometry | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
AKR4C7 | - |
Zea mays |
aldose reductase | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Zea mays |