Protein Variants | Comment | Organism |
---|---|---|
R418H | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Tritrichomonas suis |
R418K | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Tritrichomonas suis |
R418Q | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Tritrichomonas suis |
T312A | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Tritrichomonas suis |
Y419F | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Tritrichomonas suis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetics, pre-steady-state kinetics, mechanistic rate constants, wild-type enzyme and mutant enzymes, overview | Tritrichomonas suis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Tritrichomonas suis | - |
xanthosine 5'-phosphate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tritrichomonas suis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + NAD+ + H2O = XMP + NADH + H+ | covalent enzyme reaction intermediate, hydrolysis of enzyme-XMP is rate-limiting and requires the adoption of the closed conformation, Arg418 is involved in the reaction mechanism as catalytic base, Thr321 is involved in both hydride transfer and hydrolysis | Tritrichomonas suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Tritrichomonas suis | xanthosine 5'-phosphate + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IMPDH | - |
Tritrichomonas suis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Tritrichomonas suis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tritrichomonas suis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of wild-type enzyme and mutant Y419F, overview | Tritrichomonas suis |
5.5 | 9 | - |
Tritrichomonas suis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Tritrichomonas suis | |
NADH | - |
Tritrichomonas suis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | multi-inhibitor kinetics | Tritrichomonas suis |