Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | final concentration of 0.05% stimulates 50% | Klebsiella aerogenes | |
glutathione | level for maximum activity: 2-4 mM | Klebsiella aerogenes |
General Stability | Organism |
---|---|
activity is maintained when stored for 3 weeks at -20°C, during which it can be thawed and frozen twice and kept at 2°C for two 24-h periods | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-Chloropurine ribonucleoside 5'-phosphate | inosine 5'-phosphate and guanosine 5'-phosphate retard inactivation | Klebsiella aerogenes | |
6-thioinosine 5'-phosphate | complete inactivation after 10 min preincubation at a concentration of 0.001 mM; in absence of glutathione | Klebsiella aerogenes | |
glutathione | above 4 mM | Klebsiella aerogenes | |
GMP | competitive with respect to IMP | Klebsiella aerogenes | |
K+ | above 0.1 M | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
6-thio-IMP | pH 8.1 | Klebsiella aerogenes | |
0.021 | - |
inosine 5'-phosphate | 6-thioinosine 5'-phosphate, constant in pH-range 6.5-8.2, sharp decrease at higher pH | Klebsiella aerogenes | |
1.1 | - |
NAD+ | - |
Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requirement of 0.1 M for maximal activity | Klebsiella aerogenes | |
NH4+ | required | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.2 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
2°C, 50% loss of activity after 2-3 days in dilutions with water | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-thio-IMP + NAD+ + H2O | - |
Klebsiella aerogenes | 6-thio-XMP + NADH + H+ | - |
r | |
6-thioinosine 5'-phosphate + NAD+ + H2O | - |
Klebsiella aerogenes | 6-thioxanthosine 5'-phosphate + NADH | - |
? | |
inosine 5'-phosphate + NAD+ + H2O | - |
Klebsiella aerogenes | xanthosine 5'-phosphate + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella aerogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
GMP | - |
Klebsiella aerogenes | |
0.14 | - |
6-thio-IMP | - |
Klebsiella aerogenes |