Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Penicillium camemberti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
galacturonate | - |
Penicillium camemberti | |
glucuronate | glucuronate shows minor substrate inhibition | Penicillium camemberti | |
L-gulonate | competitive inhibitor | Penicillium camemberti | |
L-gulono-1,4-lactone | competitive inhibitor | Penicillium camemberti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | with D-glucuronic acid as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.4 | - |
NADH | at pH 7.5 and 25°C | Penicillium camemberti | |
0.95 | - |
D-galacturonic acid | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
3.15 | - |
D-galacturonic acid | with NADH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
3.3 | - |
L-galactonate | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
3.34 | - |
D-glucuronic acid | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
4.7 | - |
L-Gulonic acid | with NADP+ as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
6.3 | - |
D-glucuronic acid | with NADH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
7.1 | - |
D-glucurono-3,6-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
8.1 | - |
L-galactono-1,4-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
9.5 | - |
L-gulono-1,4-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonic acid + NADPH | Penicillium camemberti | - |
L-galactonate + NADP+ | - |
? | |
D-glucuronic acid + NADPH | Penicillium camemberti | - |
L-gulonic acid + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium camemberti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Penicillium camemberti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonic acid + NADPH | - |
Penicillium camemberti | L-galactonate + NADP+ | - |
? | |
D-glucuronic acid + NADH | - |
Penicillium camemberti | L-gulonic acid + NAD+ | - |
? | |
D-glucuronic acid + NADPH | - |
Penicillium camemberti | L-gulonic acid + NADP+ | - |
? | |
D-glucurono-3,6-lactone + NADPH + H+ | - |
Penicillium camemberti | L-gulono-1,4-lactone + NADP+ | - |
? | |
L-galactonate + NADP+ | - |
Penicillium camemberti | D-galacturonic acid + NADPH | - |
? | |
L-galactono-1,4-lactone + NADPH | - |
Penicillium camemberti | ? | - |
? | |
L-gulonic acid + NAD+ | - |
Penicillium camemberti | D-glucuronic acid + NADH | - |
? | |
L-gulono-1,4-lactone + NADPH | - |
Penicillium camemberti | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-GalUA oxidoreductase | - |
Penicillium camemberti |
galacturonate oxidoreductase | - |
Penicillium camemberti |
Gor | - |
Penicillium camemberti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | the enzyme has low thermal stability, with at KT0.5 of 37°C, and complete unfolding after 1 h incubation at 40°C | Penicillium camemberti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
L-galactono-1,4-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.41 | - |
L-gulono-1,4-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.75 | - |
L-galactonate | with NADP+ as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.97 | - |
L-Gulonic acid | with NADP+ as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
2.3 | - |
D-glucurono-3,6-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
7.4 | - |
NADH | at pH 7.5 and 25°C | Penicillium camemberti | |
7.6 | - |
D-glucuronic acid | with NADH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
9.6 | - |
NADPH | with D-glucuronic acid as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
13 | - |
D-glucuronic acid | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
13.6 | - |
D-galacturonic acid | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
18.5 | - |
D-galacturonic acid | with NADH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Penicillium camemberti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 50% activity between pH 6.0 and 9.0 | Penicillium camemberti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Penicillium camemberti | |
NADPH | preferred cofactor | Penicillium camemberti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
L-gulonate | at pH 7.5 and 25°C | Penicillium camemberti | |
7.2 | - |
L-gulono-1,4-lactone | at pH 7.5 and 25°C | Penicillium camemberti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-gulono-1,4-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.05 | - |
L-galactono-1,4-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.2 | - |
L-Gulonic acid | with NADP+ as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.23 | - |
L-galactonate | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
0.32 | - |
D-glucurono-3,6-lactone | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
1.2 | - |
D-glucuronic acid | with NADH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
3.89 | - |
D-glucuronic acid | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
5.87 | - |
D-galacturonic acid | with NADH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
14.3 | - |
D-galacturonic acid | with NADPH as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti | |
19 | - |
NADH | at pH 7.5 and 25°C | Penicillium camemberti | |
1655 | - |
NADPH | with D-glucuronic acid as cosubstrate, at pH 7.5 and 25°C | Penicillium camemberti |