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Literature summary for 1.1.1.201 extracted from

  • Edenharder, R.; Pfutzner, A.; Hammann, R.
    Characterization of NAD-dependent 3alpha- and 3beta-hydroxysteroid dehydrogenase and of NADP-dependent 7beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus (1989), Biochim. Biophys. Acta, 1004, 230-238.
    View publication on PubMed

General Stability

General Stability Organism
presence of reducing agents protects against thermal denaturation Blautia producta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
3alpha-hydroxy-7-keto-5beta-cholanoic acid
-
Blautia producta
0.07
-
3,12-diketocholanoic acid
-
Blautia producta
0.087
-
3,7-Diketo-5beta-cholanoic acid
-
Blautia producta
0.123
-
3,7,12-Triketo-5beta-cholanoic acid
-
Blautia producta
0.124
-
3alpha-Hydroxy-7,12-diketo-5beta-cholanoic acid
-
Blautia producta
0.142
-
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid
-
Blautia producta
0.145
-
3,7,12-triketo-5beta-cholanoyl taurine
-
Blautia producta

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Blautia producta 5622
-
membrane
-
Blautia producta 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
gel filtration Blautia producta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,7,12-triketo-5beta-cholanoic acid + NADPH Blautia producta
-
7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+
-
?
3,7,12-triketo-5beta-cholanoyl taurine + NADPH Blautia producta
-
7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+
-
?
3,7-diketo-5beta-cholanoic acid + NADPH Blautia producta
-
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
-
?
3,7-diketo-5beta-cholanoic acid + NADPH Blautia producta K2/25-1
-
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH Blautia producta
-
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH Blautia producta K2/25-1
-
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Blautia producta ursodeoxycholic acid 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Blautia producta K2/25-1 ursodeoxycholic acid 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH Blautia producta
-
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH Blautia producta K2/25-1
-
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?
7,12-diketo-5beta-cholanoic acid + NADPH Blautia producta
-
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?
7,12-diketo-5beta-cholanoic acid + NADPH Blautia producta K2/25-1
-
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Blautia producta
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Blautia producta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,12-diketocholanoic acid + NADP+
-
Blautia producta ?
-
r
3,7,12-triketo-5beta-cholanoic acid + NADPH
-
Blautia producta 7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+
-
?
3,7,12-triketo-5beta-cholanoyl taurine + NADPH
-
Blautia producta 7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+
-
?
3,7-diketo-5beta-cholanoic acid + NADPH
-
Blautia producta 7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
-
?
3,7-diketo-5beta-cholanoic acid + NADPH
-
Blautia producta K2/25-1 7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Blautia producta 3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
Blautia producta K2/25-1 3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ ursodeoxycholic acid Blautia producta 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ ursodeoxycholic acid Blautia producta K2/25-1 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
?
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
-
Blautia producta 3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
-
Blautia producta K2/25-1 3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?
7,12-diketo-5beta-cholanoic acid + NADPH
-
Blautia producta 7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?
7,12-diketo-5beta-cholanoic acid + NADPH
-
Blautia producta K2/25-1 7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
50% loss of activity within 60 min Blautia producta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Blautia producta

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Blautia producta