Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.2 extracted from

  • Patel, J.M.; Musa, M.M.; Rodriguez, L.; Sutton, D.A.; Popik, V.V.; Phillips, R.S.
    Mutation of Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase at Trp-110 affects stereoselectivity of aromatic ketone reduction (2014), Org. Biomol. Chem., 12, 5905-5910.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermoanaerobacter ethanolicus

Protein Variants

Protein Variants Comment Organism
W110A 99.9% conversion of phenylacetone to 84.1% S-product Thermoanaerobacter ethanolicus
W110G 99.9% conversion of phenylacetone to 79% S-product, 95.8% conversion of 1-phenyl-2-butanone to 91.6% S-product, 99.1% conversion of 4-phenyl-2-butanone to 70.5% S-product Thermoanaerobacter ethanolicus
W110I 99.9% conversion of phenylacetone to 99.9% S-product, 99.4% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
W110L 99.9% conversion of phenylacetone to 99.9% S-product, 98.9% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.2% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
W110M 99.9% conversion of phenylacetone to 99.9% S-product, 97.3% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.3% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
W110Q 99.9% conversion of phenylacetone to 99.9% S-product, 83.5% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus
W110V 99.9% conversion of phenylacetone to 99.9% S-product, 99.2% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenyl-2-propanol + NADP+
-
Thermoanaerobacter ethanolicus phenylacetone + NADPH + H+
-
?
(S)-1-phenyl-2-propanol + NADP+
-
Thermoanaerobacter ethanolicus phenylacetone + NADPH + H+
-
r
1-phenyl-2-butanone + NADPH + H+
-
Thermoanaerobacter ethanolicus (S)-1-phenyl-2-butanol + NADP+
-
?
4-phenyl-2-butanone + NADPH + H+
-
Thermoanaerobacter ethanolicus (S)-4-phenyl-2-butanol + NADP+
-
?
phenylacetone + NADPH + H+
-
Thermoanaerobacter ethanolicus (S)-1-phenyl-2-propanol + NADP+
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
(R)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.025
-
(R)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.045
-
(R)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.46
-
(R)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.56
-
(R)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.65
-
(S)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.2
-
(R)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.4
-
(R)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
2.3
-
(S)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
4.5
-
(S)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
17.8
-
(S)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
18.8
-
(S)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
31.1
-
(S)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
38.6
-
(S)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Thermoanaerobacter ethanolicus
NADPH
-
Thermoanaerobacter ethanolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0069
-
(R)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.024
-
(R)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.121
-
(R)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.188
-
(R)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.284
-
(R)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.336
-
(R)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.551
-
(S)-1-phenyl-2-propanol mutant W110Q, pH 8.9, 50°C Thermoanaerobacter ethanolicus
0.639
-
(R)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
1.99
-
(S)-1-phenyl-2-propanol mutant W110M, pH 8.9, 50°C Thermoanaerobacter ethanolicus
2.51
-
(S)-1-phenyl-2-propanol mutant W110L, pH 8.9, 50°C Thermoanaerobacter ethanolicus
4.935
-
(S)-1-phenyl-2-propanol mutant W110A, pH 8.9, 50°C Thermoanaerobacter ethanolicus
5.8
-
(S)-1-phenyl-2-propanol mutant W110G, pH 8.9, 50°C Thermoanaerobacter ethanolicus
15.2
-
(S)-1-phenyl-2-propanol mutant W110I, pH 8.9, 50°C Thermoanaerobacter ethanolicus
45.3
-
(S)-1-phenyl-2-propanol mutant W110V, pH 8.9, 50°C Thermoanaerobacter ethanolicus