Cloned (Comment) | Organism |
---|---|
- |
Thermoanaerobacter ethanolicus |
Protein Variants | Comment | Organism |
---|---|---|
W110A | 99.9% conversion of phenylacetone to 84.1% S-product | Thermoanaerobacter ethanolicus |
W110G | 99.9% conversion of phenylacetone to 79% S-product, 95.8% conversion of 1-phenyl-2-butanone to 91.6% S-product, 99.1% conversion of 4-phenyl-2-butanone to 70.5% S-product | Thermoanaerobacter ethanolicus |
W110I | 99.9% conversion of phenylacetone to 99.9% S-product, 99.4% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product | Thermoanaerobacter ethanolicus |
W110L | 99.9% conversion of phenylacetone to 99.9% S-product, 98.9% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.2% conversion of 4-phenyl-2-butanone to 99.9% S-product | Thermoanaerobacter ethanolicus |
W110M | 99.9% conversion of phenylacetone to 99.9% S-product, 97.3% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.3% conversion of 4-phenyl-2-butanone to 99.9% S-product | Thermoanaerobacter ethanolicus |
W110Q | 99.9% conversion of phenylacetone to 99.9% S-product, 83.5% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product | Thermoanaerobacter ethanolicus |
W110V | 99.9% conversion of phenylacetone to 99.9% S-product, 99.2% conversion of 1-phenyl-2-butanone to 99.9% S-product, 99.1% conversion of 4-phenyl-2-butanone to 99.9% S-product | Thermoanaerobacter ethanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-phenyl-2-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | phenylacetone + NADPH + H+ | - |
? | |
(S)-1-phenyl-2-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | phenylacetone + NADPH + H+ | - |
r | |
1-phenyl-2-butanone + NADPH + H+ | - |
Thermoanaerobacter ethanolicus | (S)-1-phenyl-2-butanol + NADP+ | - |
? | |
4-phenyl-2-butanone + NADPH + H+ | - |
Thermoanaerobacter ethanolicus | (S)-4-phenyl-2-butanol + NADP+ | - |
? | |
phenylacetone + NADPH + H+ | - |
Thermoanaerobacter ethanolicus | (S)-1-phenyl-2-propanol + NADP+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
(R)-1-phenyl-2-propanol | mutant W110L, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.025 | - |
(R)-1-phenyl-2-propanol | mutant W110Q, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.045 | - |
(R)-1-phenyl-2-propanol | mutant W110M, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.46 | - |
(R)-1-phenyl-2-propanol | mutant W110I, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.56 | - |
(R)-1-phenyl-2-propanol | mutant W110A, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.65 | - |
(S)-1-phenyl-2-propanol | mutant W110L, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
1.2 | - |
(R)-1-phenyl-2-propanol | mutant W110V, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
1.4 | - |
(R)-1-phenyl-2-propanol | mutant W110G, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
2.3 | - |
(S)-1-phenyl-2-propanol | mutant W110Q, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
4.5 | - |
(S)-1-phenyl-2-propanol | mutant W110M, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
17.8 | - |
(S)-1-phenyl-2-propanol | mutant W110G, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
18.8 | - |
(S)-1-phenyl-2-propanol | mutant W110I, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
31.1 | - |
(S)-1-phenyl-2-propanol | mutant W110A, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
38.6 | - |
(S)-1-phenyl-2-propanol | mutant W110V, pH 8.9, 50°C | Thermoanaerobacter ethanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermoanaerobacter ethanolicus | |
NADPH | - |
Thermoanaerobacter ethanolicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0069 | - |
(R)-1-phenyl-2-propanol | mutant W110Q, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.024 | - |
(R)-1-phenyl-2-propanol | mutant W110L, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.121 | - |
(R)-1-phenyl-2-propanol | mutant W110M, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.188 | - |
(R)-1-phenyl-2-propanol | mutant W110I, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.284 | - |
(R)-1-phenyl-2-propanol | mutant W110A, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.336 | - |
(R)-1-phenyl-2-propanol | mutant W110V, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.551 | - |
(S)-1-phenyl-2-propanol | mutant W110Q, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
0.639 | - |
(R)-1-phenyl-2-propanol | mutant W110G, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
1.99 | - |
(S)-1-phenyl-2-propanol | mutant W110M, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
2.51 | - |
(S)-1-phenyl-2-propanol | mutant W110L, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
4.935 | - |
(S)-1-phenyl-2-propanol | mutant W110A, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
5.8 | - |
(S)-1-phenyl-2-propanol | mutant W110G, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
15.2 | - |
(S)-1-phenyl-2-propanol | mutant W110I, pH 8.9, 50°C | Thermoanaerobacter ethanolicus | |
45.3 | - |
(S)-1-phenyl-2-propanol | mutant W110V, pH 8.9, 50°C | Thermoanaerobacter ethanolicus |