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Literature summary for 1.1.1.2 extracted from
- Novel NADP-linked alcohol-aldehyde/ketone oxidoreductase in thermophilic ethanologenic bacteria (1981), Biochem. J., 195, 183-190.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 1 mM, complete inactivation | Thermoanaerobacter brockii |  |
| additional information | not inhibitory: EDTA (5 mM), MgCl2 (10 mM), CaCl2 (1O mM), CuCl2 (1 mM) and ZnCl2 (1 mM) | Thermoanaerobacter brockii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
Butanone | pH 7.8, 40°C | Thermoanaerobacter brockii | |
| 0.31 | - |
butan-2-ol | pH 7.8, 40°C | Thermoanaerobacter brockii | |
| 0.7 | - |
acetone | pH 7.8, 40°C | Thermoanaerobacter brockii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Thermoanaerobacter brockii |
| 150000 | - |
gel filtration | Thermoanaerobacter brockii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter brockii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoanaerobacter brockii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetone + NADPH + H+ | about 13% of the activity with butan-2-ol | Thermoanaerobacter brockii | propan-2-ol + NADP+ | - |
? | |
| butan-2-ol + NADP+ | good substrate | Thermoanaerobacter brockii | butanone + NADPH + H+ | - |
r | |
| butanone + NADPH + H+ | - |
Thermoanaerobacter brockii | butan-2-ol + NADP+ | - |
r | |
| cyclohexanol + NADP+ | about 15% of the activity with butan-2-ol | Thermoanaerobacter brockii | cyclohexanone + NADPH + H+ | - |
? | |
| additional information | activity of the reversible enzyme is highest for secondary alcohols and decreases in order of acetaldehyde, linear ketones, cyclic ketones and primary alcohols. It is active on five- to seven-membered ring ketones but not with cyclo-octanone as substrate | Thermoanaerobacter brockii | ? | - |
? | |
| pent-1-en-3-ol + NADP+ | good substrate | Thermoanaerobacter brockii | pent-1-en-3-one + NADPH + H+ | - |
? | |
| propan-2-ol + NADP+ | good substrate | Thermoanaerobacter brockii | acetone + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Thermoanaerobacter brockii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
70 min, no loss of activity | Thermoanaerobacter brockii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Thermoanaerobacter brockii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | more than 50% of maximum activity | Thermoanaerobacter brockii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NAD(H) | Thermoanaerobacter brockii | |
| NADPH | - |
Thermoanaerobacter brockii | |
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Release 2023.1 (January 2023)
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