Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Penicillium citrinum |
Protein Variants | Comment | Organism |
---|---|---|
K245R | mutation leads to improved thermostability | Penicillium citrinum |
L54Q | mutant retains 62% of its initial activity after heat treatment at 30°C for 6 h and mutation confers improved enantioselectivity | Penicillium citrinum |
L54Q/K245R/N271D | mutant displays improved thermostability and mutation confers improved enantioselectivity | Penicillium citrinum |
L54Q/R104C | mutation leads to improved thermostability and enantioselectivity | Penicillium citrinum |
N271D | mutation leads to improved thermostability | Penicillium citrinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
methyl 4-bromo-3-oxobutanoate | wild-type, pH 6.5, 30°C | Penicillium citrinum | |
1.2 | - |
methyl 4-bromo-3-oxobutanoate | mutant L54Q, pH 6.5, 30°C | Penicillium citrinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium citrinum | - |
- |
- |
Penicillium citrinum IFO 4631 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Penicillium citrinum | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Penicillium citrinum IFO 4631 | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
methyl 4-bromo-3-oxobutanoate + NADPH + H+ | - |
Penicillium citrinum | methyl (S)-4-bromo-3-hydroxybutanoate + NADP+ | - |
? | |
methyl 4-bromo-3-oxobutanoate + NADPH + H+ | - |
Penicillium citrinum IFO 4631 | methyl (S)-4-bromo-3-hydroxybutanoate + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
6 h, 15% residual activity for wild-type | Penicillium citrinum |
40 | - |
2 h, remaining activity is 1.7% for wild-type, 3.8% for mutant K245R, 10.1% for mutant N271D, and 74.9% for mutant L54Q/R104C | Penicillium citrinum |
45 | - |
7 h, complete loss of activity for wild-type, 69.9% residual activity for mutant L54Q/K245R/N271D, 18.1% for mutant L54Q/R104C/K245R/N271D | Penicillium citrinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Penicillium citrinum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1160 | - |
methyl 4-bromo-3-oxobutanoate | mutant L54Q, pH 6.5, 30°C | Penicillium citrinum | |
1420 | - |
methyl 4-bromo-3-oxobutanoate | wild-type, pH 6.5, 30°C | Penicillium citrinum |