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Literature summary for 1.1.1.2 extracted from

  • Fossati, E.; Polentini, F.; Carrea, G.; Riva, S.
    Exploitation of the alcohol dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid (2006), Biotechnol. Bioeng., 93, 1216-1220.
    View publication on PubMed
Search for more literature for 1.1.1.2

Application

Application Comment Organism
analysis the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Levilactobacillus brevis
analysis the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Thermoanaerobacter brockii

Protein Variants

Protein Variants Comment Organism
additional information evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Levilactobacillus brevis
additional information evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview Thermoanaerobacter brockii

Inhibitors

Inhibitors Comment Organism Structure
12-ketochenodeoxycholic acid inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially Levilactobacillus brevis
acetone at high concentrations of e.g. 3.4 M Levilactobacillus brevis
acetone at high concentrations of e.g. 3.4 M Thermoanaerobacter brockii
cholic acid inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-propanol + NADP+ Levilactobacillus brevis
-
 acetone + NADPH
-
 r
2-propanol + NADP+ Thermoanaerobacter brockii
-
 acetone + NADPH
-
 r

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis
-
-
-
Thermoanaerobacter brockii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Levilactobacillus brevis
-
commercial preparation
-
 Thermoanaerobacter brockii
-

Storage Stability

Storage Stability Organism
1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, 51% and 46% residual activity, respectively Levilactobacillus brevis
1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, quite stable Thermoanaerobacter brockii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-propanol + NADP+
-
 Levilactobacillus brevis acetone + NADPH
-
 r
2-propanol + NADP+
-
 Thermoanaerobacter brockii acetone + NADPH
-
 r

Synonyms

Synonyms Comment Organism
ADH
-
 Levilactobacillus brevis
ADH
-
 Thermoanaerobacter brockii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Levilactobacillus brevis
22
-
 assay at room temperature Thermoanaerobacter brockii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Levilactobacillus brevis
8
-
 assay at Thermoanaerobacter brockii

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Levilactobacillus brevis
NADP+ dependent on Thermoanaerobacter brockii
NADPH dependent on Levilactobacillus brevis
NADPH dependent on Thermoanaerobacter brockii