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Literature summary for 1.1.1.2 extracted from
- Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: relevance in aldehyde reduction (2002), Biochem. J., 361, 163-172.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | gene deletion mutant and combined gene deletion mutant lacking both ADH and ADH7, both are viable and show similar growth curves as wild-type | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
NADPH | pH 7.0, 25°C | Saccharomyces cerevisiae |  |
| 0.013 | - |
NADP+ | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 0.029 | - |
NADPH | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.043 | - |
cinnamaldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.048 | - |
3-Methylbutanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.049 | - |
pentanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.058 | - |
veratraldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.06 | - |
pentanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.061 | - |
NADP+ | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 0.073 | - |
veratraldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.08 | - |
cinnamyl alcohol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 0.11 | - |
cinnamyl alcohol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.129 | - |
3-Methylbutanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.152 | - |
hexanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.172 | - |
cinnamaldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.436 | - |
cinnamyl alcohol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 0.7 | - |
cinnamyl alcohol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 0.99 | - |
1-Pentanol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 1.61 | - |
3-methyl-1-butanol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 2.2 | - |
Phenylethanol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 4.6 | - |
3-methyl-1-butanol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 9.1 | - |
Hexanol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Saccharomyces cerevisiae |
| 71000 | - |
gel filtration | Saccharomyces cerevisiae |
| 81000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | - |
ADH7 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90 | - |
pH 7.0, 25°C | Saccharomyces cerevisiae |
| 183 | - |
25°C, pH 7.0 | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% v/v glycerol, stable for at least one month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E)-4-hydroxynon-2-enal + NADPH | 30% of activity with cinnamaldehyde | Saccharomyces cerevisiae | (2E)-non-2-ene-1,4-diol + NADP+ | - |
r | |
| (2S)-methyl-1-butanol + NADP+ | 39% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | (2S)-methylbutanal + NADPH | - |
r | |
| 1-butanol + NADP+ | 42% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | butanal + NADPH | - |
r | |
| 1-hexanol + NADP+ | 45% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | hexanal + NADPH | - |
r | |
| 1-octanol + NADP+ | 30% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | octanal + NADPH | - |
r | |
| 1-pentanol + NADP+ | 46% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | pentanal + NADPH | - |
r | |
| 1-propanol + NADP+ | 16% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | propanal + NADPH | - |
r | |
| 2-(S)-methylbutanol + NADP+ | 7% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | (2S)-2-methylbutanal + NADPH | - |
r | |
| 2-butanol + NADP+ | 2% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | 2-butanone + NADPH | - |
r | |
| 2-butanol + NADP+ | 1% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | butanone + NADPH | - |
r | |
| 2-methyl-1-propanol + NADP+ | 28% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | 2-methylpropanal + NADPH | - |
r | |
| 2-methylbutanal + NADPH | 89% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 2-methyl-1-butanol + NADP+ | - |
r | |
| 2-methylbutanal + NADPH | 92% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 2-methyl-1-butanol + NADP+ | - |
r | |
| 2-methylpropanal + NADPH | 63% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 2-methyl-1-propanol + NADP+ | - |
r | |
| 2-methylpropanal + NADPH | 67% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 2-methyl-1-propanol + NADP+ | - |
r | |
| 2-methylpropanol + NADP+ | 1% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | 2-methylpropanal + NADPH | - |
r | |
| 2-phenylethanol + NADP+ | 23% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | phenylacetaldehyde + NADPH | - |
r | |
| 3-methyl-1-butanol + NADP+ | 40% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | 3-methylbutanal + NADPH | - |
r | |
| 3-methylbutanal + NADPH | 79% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 3-methyl-1-butanol + NADP+ | - |
r | |
| 3-methylbutanal + NADPH + H+ | 84% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 3-methyl-1-butanol + NADP+ | - |
r | |
| 3-methylbutanol + NADP+ | 15% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | 3-methylbutanal + NADPH | - |
r | |
| anisaldehyde + NADPH | 72% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 4-methoxybenzyl alcohol + NADP+ | - |
r | |
| anisaldehyde + NADPH | 83% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 4-methoxybenzyl alcohol + NADP+ | - |
r | |
| benzaldehyde + NADPH | 45% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | benzyl alcohol + NADP+ | - |
r | |
| benzaldehyde + NADPH | 72% of activity with cinnamaldehyde | Saccharomyces cerevisiae | benzyl alcohol + NADP+ | - |
r | |
| benzyl alcohol + NADP+ | 45% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | benzaldehyde + NADPH | - |
r | |
| benzyl alcohol + NADP+ | 78% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | benzaldehyde + NADPH | - |
r | |
| butanal + NADPH + H+ | 19% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 1-butanol + NADP+ | - |
r | |
| butanal + NADPH + H+ | 57% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 1-butanol + NADP+ | - |
r | |
| butane-1,2-diol + NADP+ | 2% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | butanal-2-ol + NADPH | - |
r | |
| butane-1,3-diol + NADP+ | 5% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | butanal-3-ol + NADPH | - |
r | |
| butane-1,4-diol + NADP+ | 8% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | butanal-4-ol + NADPH | - |
r | |
| butanol + NADP+ | 24% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | butanal + NADPH | - |
r | |
| cinnamaldehyde + NADPH | - |
Saccharomyces cerevisiae | cinnamyl alcohol + NADP+ | - |
r | |
| cinnamyl alcohol + NADP+ | - |
Saccharomyces cerevisiae | cinnamaldehyde + NADPH | - |
r | |
| coniferaldehyde + NADPH | 10% of activity with cinnamaldehyde | Saccharomyces cerevisiae | coniferyl alcohol + NADP+ | - |
r | |
| coniferaldehyde + NADPH | 15% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | coniferyl alcohol + NADP+ | - |
r | |
| coniferyl alcohol + NADP+ | 27% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | coniferylaldehyde + NADPH + H+ | - |
r | |
| coniferyl alcohol + NADP+ | 7% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | coniferaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | 2% activity compared to cinnamyl alcohol | Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | 4% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
| furfural + NADPH | 27% of activity with cinnamaldehyde | Saccharomyces cerevisiae | furfuryl alcohol + NADP+ | - |
r | |
| furfural + NADPH | 54% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | furfuryl alcohol + NADP+ | - |
r | |
| heptanal + NADPH | 86% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 1-heptanol + NADP+ | - |
r | |
| heptanal + NADPH | 88% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 1-heptanol + NADP+ | - |
r | |
| hexanal + NADPH + H+ | 88% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 1-hexanol + NADP+ | - |
r | |
| hexanal + NADPH + H+ | 89% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 1-hexanol + NADP+ | - |
r | |
| hexanol + NADP+ | 154% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | hexanal + NADPH | - |
r | |
| additional information | no subbstrate: acetone, methylglyoxal, cyclohexanone | Saccharomyces cerevisiae | ? | - |
? | |
| octanal + NADPH + H+ | 59% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 1-octanol + NADP+ | - |
r | |
| octanal + NADPH + H+ | 84% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 1-octanol + NADP+ | - |
r | |
| octanol + NADP+ | 52% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | octanal + NADPH | - |
r | |
| pentanal + NADPH | 109% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 1-pentanol + NADP+ | - |
r | |
| pentanal + NADPH | 153% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 1-pentanol + NADP+ | - |
r | |
| pentanol + NADP+ | 89% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | pentanal + NADPH | - |
r | |
| phenylacetaldehyde + NADPH + H+ | 90% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 2-phenylethanol + NADP+ | - |
r | |
| propanal + NADPH + H+ | 21% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 1-propanol + NADP+ | - |
r | |
| propanal + NADPH + H+ | 24% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 1-propanol + NADP+ | - |
r | |
| propanol + NADP+ | 7% of activity with cinnamyl alcohol | Saccharomyces cerevisiae | propanal + NADPH | - |
r | |
| sinapaldehyde + NADPH + H+ | 3% of activity with cinnamaldehyde | Saccharomyces cerevisiae | sinapyl alcohol + NADP+ | - |
r | |
| trans-2-nonenal + NADPH | 33% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | trans-2-nonen-1-ol + NADP+ | - |
r | |
| trans-2-nonenal + NADPH | 39% of activity with cinnamaldehyde | Saccharomyces cerevisiae | trans-2-nonenol + NADP+ | - |
r | |
| vanillin + NADPH | 57% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 4-hydroxy-3-methoxybenzyl alcohol + NADP+ | - |
r | |
| vanillin + NADPH | 84% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 4-hydroxy-3-methoxybenzyl alcohol + NADP+ | - |
r | |
| veratraldehyde + NADPH | 79% activity compared to cinnamaldehyde | Saccharomyces cerevisiae | 3,4-dimethoxybenzyl alcohol + NADP+ | - |
r | |
| veratraldehyde + NADPH | 82% of activity with cinnamaldehyde | Saccharomyces cerevisiae | 3,4-dimethoxybenzyl alcohol + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.7 | - |
Hexanol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 14.8 | - |
cinnamyl alcohol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 70 | - |
cinnamyl alcohol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 173 | - |
3-methyl-1-butanol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 263 | - |
veratraldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 296 | - |
3-Methylbutanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 307 | - |
cinnamaldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 359 | - |
hexanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 378 | - |
pentanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 918 | - |
1-Pentanol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 1132 | - |
Phenylethanol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 1622 | - |
cinnamyl alcohol | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 2866 | - |
3-methyl-1-butanol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 3404 | - |
cinnamyl alcohol | pH 10.0, 25°C | Saccharomyces cerevisiae | |
| 6000 | - |
veratraldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 7913 | - |
cinnamaldehyde | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 9581 | - |
3-Methylbutanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 11920 | - |
pentanal | pH 7.0, 25°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
optimum for reduction reaction, assay of reduction and oxidation at | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | oxidation reaction, increasing activity from pH 6.0 to 11.0 | Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | isoelectric focusing | - |
4.9 |
| Saccharomyces cerevisiae | and 6.2, isoelectric focusing | - |
6 |
| Saccharomyces cerevisiae | and 6.4, isoelectric focusing | - |
6 |
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