Cloned (Comment) | Organism |
---|---|
gene cloned and sequenced | Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADP+ | - |
Thermococcus sp. | |
0.12 | 0.13 | acetaldehyde | - |
Thermococcus sp. | |
1.4 | - |
butanol | - |
Thermococcus sp. | |
1.5 | - |
NAD+ | - |
Thermococcus sp. | |
10 | - |
ethanol | - |
Thermococcus sp. | |
12 | - |
n-Propanol | - |
Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46700 | - |
4 * 46700 | Thermococcus sp. |
158000 | - |
gel filtration | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
- |
- |
Clostridium acetobutylicum | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
yeast | - |
Thermococcus sp. | - |
- |
- |
Thermococcus sp. AN1 | - |
- |
- |
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Thermococcus sp. |
Storage Stability | Organism |
---|---|
4°C half-life 6-7 days when stored aerobically | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-octanol + NADP+ | - |
Thermococcus sp. | octanal + NADPH | - |
r | |
1-pentanol + NADP+ | - |
Thermococcus sp. | n-pentanal + NADPH | - |
r | |
acetaldehyde + NADPH + H+ | - |
Thermococcus sp. | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Thermococcus sp. AN1 | ethanol + NADP+ | - |
? | |
benzyl alcohol + NAD+ | - |
Thermococcus sp. | benzaldehyde + NADH + H+ | - |
r | |
ethanol + NADP+ | - |
Escherichia coli | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Zymomonas mobilis | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Clostridium acetobutylicum | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Thermococcus sp. | acetaldehyde + NADPH + H+ | - |
r | |
ethanol + NADP+ | - |
Thermococcus sp. AN1 | acetaldehyde + NADPH + H+ | - |
r | |
additional information | methanol, isopropanol and glycerol are no substrates | Thermococcus sp. | ? | - |
? | |
additional information | methanol, isopropanol and glycerol are no substrates | Thermococcus sp. AN1 | ? | - |
? | |
n-butanol + NADP+ | - |
Zymomonas mobilis | butyraldehyde + NADPH | - |
r | |
n-butanol + NADP+ | - |
Thermococcus sp. | butyraldehyde + NADPH | - |
r | |
n-butanol + NADP+ | - |
Thermococcus sp. AN1 | butyraldehyde + NADPH | - |
r | |
n-propanol + NADP+ | - |
Thermococcus sp. | propionaldehyde + NADPH | - |
r | |
n-propanol + NADP+ | - |
Thermococcus sp. AN1 | propionaldehyde + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46700 | Thermococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
ethanol as substrate | Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life of 16 min | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | ethanol as substrate | Thermococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermococcus sp. |