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Literature summary for 1.1.1.2 extracted from
- Purification and characterization of NADP-specific alcohol dehydrogenase and glutamate dehydrogenase from the hyperthermophilic archaeon Thermococcus litoralis (1994), Appl. Environ. Microbiol., 60, 562-568.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Thermococcus litoralis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Thermococcus litoralis | |
| NaCl | - |
Thermococcus litoralis | |
| potassium phosphate | - |
Thermococcus litoralis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
NADP+ | - |
Thermococcus litoralis | |
| 0.1 | - |
NADPH | - |
Thermococcus litoralis | |
| 0.4 | - |
acetaldehyde | - |
Thermococcus litoralis | |
| 11.1 | - |
ethanol | - |
Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, SDS-PAGE | Thermococcus litoralis |
| 175000 | - |
- |
Methanobacterium palustre |
| 200000 | - |
gel filtration | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter aceti | - |
- |
- |
| Drosophila melanogaster | - |
- |
- |
| Methanobacterium palustre | - |
- |
- |
| Methanoculleus thermophilus | - |
- |
- |
| Methanofollis liminatans | - |
- |
- |
| Saccharomyces cerevisiae | - |
yeast | - |
| Thermoanaerobacter brockii | - |
- |
- |
| Thermococcus litoralis | - |
DSM 5473 | - |
| Zymomonas mobilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetobacter aceti |
- |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.1 | - |
- |
Thermococcus litoralis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 23°C, loses all of its activity within 1 week when stored anaerobically | Thermococcus litoralis |
| 4°C, about 1 week, 50% activity lost | Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-propanediol + NADP+ | - |
Thermococcus litoralis | ? + NADPH | - |
? | |
| 1-octanol + NADP+ | - |
Thermococcus litoralis | octanal + NADPH | - |
r | |
| 1-pentanol + NADP+ | - |
Thermococcus litoralis | n-pentanal + NADPH | - |
r | |
| ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | - |
Zymomonas mobilis | acetaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | - |
Thermococcus litoralis | acetaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | - |
Thermoanaerobacter brockii | acetaldehyde + NADPH + H+ | - |
r | |
| isobutanol + NADP+ | - |
Thermococcus litoralis | isobutanal + NADPH + H+ | - |
r | |
| n-butanol + NADP+ | - |
Zymomonas mobilis | butyraldehyde + NADPH | - |
r | |
| n-butanol + NADP+ | - |
Thermococcus litoralis | butyraldehyde + NADPH | - |
r | |
| n-heptanol + NADP+ | - |
Saccharomyces cerevisiae | n-heptanal + NADPH | - |
r | |
| n-heptanol + NADP+ | - |
Thermococcus litoralis | n-heptanal + NADPH | - |
r | |
| n-hexanol + NADP+ | - |
Saccharomyces cerevisiae | n-hexanal + NADPH | - |
r | |
| n-hexanol + NADP+ | - |
Thermococcus litoralis | n-hexanal + NADPH | - |
r | |
| n-propanol + NADP+ | - |
Thermococcus litoralis | propionaldehyde + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Saccharolobus solfataricus |
| tetramer | - |
Thermoanaerobacter brockii |
| tetramer | - |
Methanobacterium palustre |
| tetramer | 4 * 48000, SDS-PAGE | Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
ethanol oxidation | Thermococcus litoralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
2 h, 50% activity lost | Thermococcus litoralis |
| 96 | - |
15 min, 50% activity lost | Thermococcus litoralis |
| 98 | - |
half-life 1-2 min | Thermoanaerobacter brockii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
ethanol oxidation | Thermococcus litoralis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Thermococcus litoralis | |
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