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Literature summary for 1.1.1.2 extracted from

  • Burdette, D.; Zeikus, J.G.
    Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase-acetyl-CoA reductive thioesterase (1994), Biochem. J., 302, 163-170.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis potential use for industrial production of ethanol by fermentation, thermophilic fermentations offer the potential to separate ethanol from continous cultures at process temperature and reduced pressure during growth Thermoanaerobacter ethanolicus
synthesis industrial ethanol production Saccharomyces cerevisiae
synthesis industrial ethanol production Zymomonas mobilis
synthesis industrial ethanol production Thermoanaerobacter ethanolicus

Cloned(Commentary)

Cloned (Comment) Organism
1° Adh cloned and characterized Escherichia coli
1° Adh cloned and characterized Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADP+ 1° Adh, ethanol as substrate Thermoanaerobacter ethanolicus
0.025
-
NADH 1° Adh, acetaldehyde as substrate Thermoanaerobacter ethanolicus
0.033
-
NADPH 2° Adh, propanone as substrate Thermoanaerobacter ethanolicus
0.049
-
NADP+ 2° Adh, butan-2-ol as substrate Thermoanaerobacter ethanolicus
0.055
-
NADPH 2° Adh, acetaldehyde as substrate Thermoanaerobacter ethanolicus
0.07
-
NADP+ 2° Adh, ethanol as substrate Thermoanaerobacter ethanolicus
0.094
-
NADPH 1°Adh, acetaldehyde as substrate Thermoanaerobacter ethanolicus
0.096
-
NAD+ 1° Adh, ethanol as substrate Thermoanaerobacter ethanolicus
0.16
-
propanone 2° Adh Thermoanaerobacter ethanolicus
0.24
-
Butanone 2° Adh Thermoanaerobacter ethanolicus
0.25
-
acetaldehyde 1° Adh Thermoanaerobacter ethanolicus
0.44
-
Butanal 1° Adh Thermoanaerobacter ethanolicus
0.5
-
acetylCoA 2° Adh Thermoanaerobacter ethanolicus
0.51
-
butan-2-ol 2° Adh Thermoanaerobacter ethanolicus
0.56
-
propan-2-ol 2° Adh Thermoanaerobacter ethanolicus
1.6
-
formaldehyde 1° Adh Thermoanaerobacter ethanolicus
2.1
-
acetaldehyde 2° Adh Thermoanaerobacter ethanolicus
2.9
-
Butanal 2° Adh Thermoanaerobacter ethanolicus
10
-
butan-1-ol 2° Adh Thermoanaerobacter ethanolicus
19
-
n-Propanol 2° Adh Thermoanaerobacter ethanolicus
23
-
n-Propanol 1° Adh Thermoanaerobacter ethanolicus
25
-
ethanol 1° Adh Thermoanaerobacter ethanolicus
34
-
n-butanol 1° Adh Thermoanaerobacter ethanolicus
66
-
ethanol 2° Adh Thermoanaerobacter ethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, 2° Adh, secondary-alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
42000
-
4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
96000
-
-
Escherichia coli
96000
-
-
Clostridium acetobutylicum
160000
-
2° Adh, gel filtration Thermoanaerobacter ethanolicus
170000
-
1° Adh, primary-alcohol dehydrogenase, gel filtration Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Clostridium acetobutylicum
-
NRRL B643
-
Clostridium acetobutylicum NRRL B643
-
NRRL B643
-
Clostridium beijerinckii
-
NRRL B592
-
Clostridium beijerinckii NRRL B592
-
NRRL B592
-
Clostridium kluyveri
-
-
-
Escherichia coli
-
-
-
Leuconostoc mesenteroides
-
-
-
Propionibacterium freudenreichii
-
-
-
Saccharomyces cerevisiae
-
yeast
-
Thermoanaerobacter brockii
-
-
-
Thermoanaerobacter ethanolicus
-
strain JW200
-
Thermoanaerobacter ethanolicus
-
39E(A.T.C.C. 33223), formerly Clostridium thermohydrosulfuricum 39E
-
Thermoanaerobacter ethanolicus JW 200
-
strain JW200
-
Thermoanaerobacter thermohydrosulfuricus
-
new name Thermoanaerobacter ethanolicus strain 39 E
-
Vibrio harveyi
-
-
-
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1° Adh and 2°Adh Thermoanaerobacter ethanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69
-
2° Adh Thermoanaerobacter ethanolicus
140
-
1° Adh Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butanol + NADP+
-
Thermoanaerobacter brockii butan-2-one + NADPH
-
r
2-butanol + NADP+ 2° Adh Thermoanaerobacter ethanolicus butan-2-one + NADPH
-
r
acetaldehyde + NADH + H+
-
Thermoanaerobacter ethanolicus ethanol + NAD+
-
r
acetaldehyde + NADH + H+
-
Thermoanaerobacter ethanolicus 39E ethanol + NAD+
-
r
acetaldehyde + NADH + H+
-
Thermoanaerobacter ethanolicus JW 200 ethanol + NAD+
-
r
acetaldehyde + NADPH + H+
-
Thermoanaerobacter ethanolicus ethanol + NADP+
-
?
acetaldehyde + NADPH + H+
-
Thermoanaerobacter ethanolicus 39E ethanol + NADP+
-
?
acetaldehyde + NADPH + H+
-
Thermoanaerobacter ethanolicus JW 200 ethanol + NADP+
-
?
acetyl-CoA + NADPH
-
Thermoanaerobacter ethanolicus acetaldehyde + CoA + NADP+
-
?
ethanol + NAD+
-
Thermoanaerobacter ethanolicus acetaldehyde + NADH + H+
-
r
ethanol + NADP+
-
Escherichia coli acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Vibrio harveyi acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Leuconostoc mesenteroides acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Zymomonas mobilis acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Thermoanaerobacter thermohydrosulfuricus acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Clostridium kluyveri acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Propionibacterium freudenreichii acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Clostridium beijerinckii acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Clostridium acetobutylicum acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Thermoanaerobacter ethanolicus acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Acetivibrio thermocellus acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Thermoanaerobacter brockii acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Clostridium acetobutylicum NRRL B643 acetaldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Clostridium beijerinckii NRRL B592 acetaldehyde + NADPH + H+
-
r
formaldehyde + NADPH
-
Thermoanaerobacter ethanolicus methanol + NADP+
-
?
formaldehyde + NADPH
-
Thermoanaerobacter ethanolicus 39E methanol + NADP+
-
?
formaldehyde + NADPH
-
Thermoanaerobacter ethanolicus JW 200 methanol + NADP+
-
?
additional information 1° Adh specific for 1°alcohols and aldehydes, no detectable activity towards ketones, 2°alcohols or acetyl-CoA Thermoanaerobacter ethanolicus ?
-
?
additional information 1° Adh specific for 1°alcohols and aldehydes, no detectable activity towards ketones, 2°alcohols or acetyl-CoA Thermoanaerobacter ethanolicus 39E ?
-
?
additional information 1° Adh specific for 1°alcohols and aldehydes, no detectable activity towards ketones, 2°alcohols or acetyl-CoA Thermoanaerobacter ethanolicus JW 200 ?
-
?
n-butanol + NADP+
-
Zymomonas mobilis butyraldehyde + NADPH
-
r
n-butanol + NADP+
-
Thermoanaerobacter ethanolicus butyraldehyde + NADPH
-
r
n-butyraldehyde + NADPH
-
Thermoanaerobacter ethanolicus n-butanol + NADP+
-
r
n-butyraldehyde + NADPH
-
Thermoanaerobacter ethanolicus 39E n-butanol + NADP+
-
r
n-butyraldehyde + NADPH
-
Thermoanaerobacter ethanolicus JW 200 n-butanol + NADP+
-
r
n-propanol + NADP+
-
Thermoanaerobacter ethanolicus propionaldehyde + NADPH
-
r
propan-2-ol + NADP+ 2° Adh Thermoanaerobacter ethanolicus propanone + NADPH
-
r

Subunits

Subunits Comment Organism
tetramer
-
Propionibacterium freudenreichii
tetramer
-
Thermoanaerobacter brockii
tetramer 4 * 40000, 2° Adh, secondary-alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
tetramer 4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus

Synonyms

Synonyms Comment Organism
2° Adh
-
Thermoanaerobacter ethanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 NAD(P)+ reduction Thermoanaerobacter ethanolicus
7
-
aldehyde-reduction Thermoanaerobacter ethanolicus
9
-
alcohol-oxidation Thermoanaerobacter ethanolicus