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Literature summary for 1.1.1.195 extracted from

  • Park, H.L.; Kim, T.L.; Bhoo, S.H.; Lee, T.H.; Lee, S.W.; Cho, M.H.
    Biochemical characterization of the rice cinnamyl alcohol dehydrogenase gene family (2018), Molecules, 23, 2659 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.195

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00266
-
 4-coumaryl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.00437
-
 coniferyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.0053
-
 cinnamyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.00642
-
 sinapyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.00819
-
 sinapyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.00936
-
 4-coumaryl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01072
-
 sinapyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01088
-
 4-coumaryl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01144
-
 coniferyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01411
-
 cinnamyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.01618
-
 coniferyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.02825
-
 cinnamyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Oryza sativa Japonica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q0JA75 cultivar Dongjin
-
Oryza sativa Japonica Group Q6ZHS4 cultivar Dongjin
-
Oryza sativa Japonica Group Q7XWU3 cultivar Dongjin
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose bead chromatography Oryza sativa Japonica Group

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa Japonica Group
-
panicle
-
 Oryza sativa Japonica Group
-
root
-
 Oryza sativa Japonica Group
-
shoot
-
 Oryza sativa Japonica Group
-
stem
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaryl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group 4-coumaryl alcohol + NADP+
-
 ?
cinnamyl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group cinnamyl alcohol + NADP+
-
 ?
coniferyl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group coniferyl alcohol + NADP+ ? ?
sinapyl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group sinapyl alcohol + NADP+ ? ?

Subunits

Subunits Comment Organism
? x * 38600, isoform CAD2, calculated from amino acid sequence Oryza sativa Japonica Group
? x * 39100, isoform CAD6, calculated from amino acid sequence Oryza sativa Japonica Group
? x * 39600, isoform CAD7, calculated from amino acid sequence Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
CAD2 isoform Oryza sativa Japonica Group
CAD6 isoform Oryza sativa Japonica Group
CAD7 isoform Oryza sativa Japonica Group
cinnamyl alcohol dehydrogenase
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.069
-
 4-coumaryl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.073
-
 sinapyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.076
-
 4-coumaryl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.128
-
 sinapyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.154
-
 coniferyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.188
-
 cinnamyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.284
-
 coniferyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
0.311
-
 cinnamyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
9.05
-
 cinnamyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
13.86
-
 4-coumaryl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
30.13
-
 coniferyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
41.19
-
 sinapyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group the expression of isoform CAD2 is induced by biotic and abiotic stresses such as Xanthomonas oryzaepv. oryzae infection and UV-irradiation up
Oryza sativa Japonica Group the expression of isoform CAD6 is induced by biotic and abiotic stresses such as Xanthomonas oryzaepv. oryzae infection and UV-irradiation up

General Information

General Information Comment Organism
physiological function isoform CAD6 is involved in developmental lignification in rice and also plays a role in the defense response against rice pathogens Oryza sativa Japonica Group
physiological function isoforms CAD2 is involved in developmental lignification in rice and also plays a role in the defense response against rice pathogens Oryza sativa Japonica Group

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.8
-
 sinapyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
7.5
-
 4-coumaryl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
9.7
-
 coniferyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
11.5
-
 cinnamyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
13.7
-
 cinnamyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
21.2
-
 sinapyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
25.3
-
 coniferyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
26.3
-
 4-coumaryl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1540.5
-
 4-coumaryl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
2369
-
 cinnamyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
5352.3
-
 sinapyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
7154.8
-
 coniferyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group