Literature summary for 1.1.1.195 extracted from

Dongming, M.; Chong, X.; Fatima, A.-G.; Hong, W.; Jinfen, Y.; Rika, J.; De-Yu, X.
Overexpression of Artemisia annua cinnamyl alcohol dehydrogenase increases lignin and coumarin and reduces artemisinin and other sesquiterpenes (2018), Front. Plant Sci., 9, 828 .

Search for more literature for 1.1.1.195

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Artemisia annua

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00194	-	sinapyl aldehyde	at pH 7.5 and 30°C	Artemisia annua
0.01076	-	coniferyl aldehyde	at pH 7.5 and 30°C	Artemisia annua
0.01105	-	4-coumaryl aldehyde	at pH 7.5 and 30°C	Artemisia annua

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Artemisia annua	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
artemisinic aldehyde + NADPH + H+	Artemisia annua	-	artemisinic alcohol + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Artemisia annua	A0A2Z5D8A5	sweet wormwood	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Artemisia annua

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Artemisia annua	-
seedling	-	Artemisia annua	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-coumaryl aldehyde + NADPH + H+	-	Artemisia annua	4-coumaryl alcohol + NADP+	-	?
artemisinic aldehyde + NADPH + H+	-	Artemisia annua	artemisinic alcohol + NADP+	-	?
coniferyl aldehyde + NADPH + H+	-	Artemisia annua	coniferyl alcohol + NADP+	-	?
sinapyl aldehyde + NADPH + H+	-	Artemisia annua	sinapyl alcohol + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
CAD	-	Artemisia annua
cinnamyl alcohol dehydrogenase	-	Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12.36	-	4-coumaryl aldehyde	at pH 7.5 and 30°C	Artemisia annua
13.82	-	coniferyl aldehyde	at pH 7.5 and 30°C	Artemisia annua
14.03	-	sinapyl aldehyde	at pH 7.5 and 30°C	Artemisia annua

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Artemisia annua

General Information

General Information	Comment	Organism
malfunction	enzyme downregulation or knockout causes a reduction of lignin, which leads to severe plant dwarfism and a decreased biomass as well as decreased plant resistance to pathogens	Artemisia annua
metabolism	key enzyme in the biosynthesis of lignin. Artemisinin, arteannuin B, and other sesquiterpenes are profiled in the leaves of enzyme-overexpressing vs. wild-type plants	Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1120	-	4-coumaryl aldehyde	at pH 7.5 and 30°C	Artemisia annua
1280	-	coniferyl aldehyde	at pH 7.5 and 30°C	Artemisia annua
7230	-	sinapyl aldehyde	at pH 7.5 and 30°C	Artemisia annua

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Release 2023.1 (January 2023)