Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Triticum aestivum |
gene TaCAD12, cloned from cv. CI12633, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of GST-tagged enzyme, overexpression of the enyzem and of GFP-tagged enzyme in Triticum aestivum cv. CI12633 | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0289 | - |
coniferyl aldehyde | recombinant GST-tagged enzyme, pH and temperature not specified in the publication | Triticum aestivum | |
0.0342 | - |
sinapyl aldehyde | recombinant GST-tagged enzyme, pH and temperature not specified in the publication | Triticum aestivum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Triticum aestivum | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | require, the TaCAD12 protein contains one alcohol dehydrogenase domain (42-157 aa) with two Zn binding sites (41-63 and 76-90 aa) and one Zn-binding dehydrogenase domain (199-323 aa) | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39430 | - |
- |
Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferyl alcohol + NADP+ | Triticum aestivum | - |
coniferyl aldehyde + NADPH + H+ | - |
r | |
sinapyl alcohol + NADP+ | Triticum aestivum | - |
sinapyl aldehyde + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
- |
- |
Triticum aestivum | A0A1D6BJ11 | sharp eyespot-resistant and -susceptive lines/cultivars | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Triticum aestivum | - |
additional information | transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines | Triticum aestivum | - |
stem | transcriptional patterns of TaCAD12 in wheat stems after Rhizoctonia cerealis inoculation analyzed by quantitative RT-PCR, overview | Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative enzyme activity in different sharp eyespot-resistant and -susceptive lines/cultivars | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferaldehyde + NADPH + H+ | - |
Triticum aestivum | coniferyl alcohol + NADP+ | - |
? | |
coniferyl alcohol + NADP+ | - |
Triticum aestivum | coniferyl aldehyde + NADPH + H+ | - |
r | |
coniferyl aldehyde + NADPH + H+ | - |
Triticum aestivum | coniferyl alcohol + NADP+ | - |
r | |
sinapyl alcohol + NADP+ | - |
Triticum aestivum | sinapyl aldehyde + NADPH + H+ | - |
r | |
sinapyl aldehyde + NADPH + H+ | - |
Triticum aestivum | sinapyl alcohol + NADP+ | - |
r | |
sinapylaldehyde + NADPH + H+ | - |
Triticum aestivum | sinapyl alcohol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39430, sequence calculation | Triticum aestivum |
More | the TaCAD12 protein contains one alcohol dehydrogenase domain (42-157 aa) with two Zn binding sites (41-63 and 76-90 aa) and one Zn-binding dehydrogenase domain (199-323 aa) with one highly conserved motif (196GLGGLG201) in CAD. The motif GLGGLG participates in binding the diophosphate group of NADP+ | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
CAD | - |
Triticum aestivum |
CAD12 | - |
Triticum aestivum |
cinnamyl alcohol dehydrogenase | - |
Triticum aestivum |
TaCAD12 | - |
Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the motif GLGGLG participates in binding the diophosphate group of NADP+ | Triticum aestivum | |
NADP+ | - |
Triticum aestivum | |
NADPH | - |
Triticum aestivum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum aestivum | sequence calculation | - |
7.39 |
Organism | Comment | Expression |
---|---|---|
Triticum aestivum | the transcriptional levels of the enzyme in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines | up |
Triticum aestivum | the enzyme is induced by infection with Rhizoctonia cerealis, a necrotrophic fungus that causes the sharp eyespot disease in wheat. Transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines | up |
General Information | Comment | Organism |
---|---|---|
evolution | TaCAD12 belongs to IV group in CAD family, phylogenetic analysis | Triticum aestivum |
malfunction | knock-down of TaCAD12 transcript significantly represses resistance of the gene-silenced wheat plants to sharp eyespot caused by Rhizoctonia cerealis, whereas TaCAD12 overexpression markedly enhances resistance of the transgenic wheat lines to sharp eyespot. Certain defense genes (Defensin, PR10, PR17c, and Chitinase1) and monolignol biosynthesis-related genes (TaCAD1, TaCCR, and TaCOMT1) are upregulated in the TaCAD12-overexpressing wheat plants but downregulated in TaCAD12-silencing plants | Triticum aestivum |
physiological function | enzyme overexpression markedly enhances resistance of the transgenic wheat lines to sharp eyespot caused by the necrotrophic fungus Rhizoctonia cerealis | Triticum aestivum |
physiological function | enzyme CAD plays a role in defense responses to necrotrophic or soil-borne pathogens in wheat | Triticum aestivum |