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Literature summary for 1.1.1.195 extracted from

  • Sattler, S.E.; Saathoff, A.J.; Haas, E.J.; Palmer, N.A.; Funnell-Harris, D.L.; Sarath, G.; Pedersen, J.F.
    A nonsense mutation in a cinnamyl alcohol dehydrogenase gene is responsible for the Sorghum brown midrib6 phenotype (2009), Plant Physiol., 150, 584-595.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.195

Cloned(Commentary)

Cloned (Comment) Organism
entire coding regions of CAD4 cloned into the pET30a vector and expressed in Escherichia coli Rossetta R2 cells Sorghum bicolor
expression in Escherichia coli Sorghum bicolor

Protein Variants

Protein Variants Comment Organism
Q132Stop mutation responsible for the bmr6 phenotype. Mutation leads to significant reduction in all three main lignin subunits, H-, G-, and S-lignin of 4.8-, 7.3-, and 17.7fold, respectively, relative to the wild type. Lignin subunits S-indene and G-indene are elevated 9.5- and 8.3fold, respectively, in bmr6 relative to the wild type Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 coniferyl alcohol
-
 Sorghum bicolor
0.0075
-
 coniferyl alcohol pH 8.8, temperature not specified in the publication Sorghum bicolor
0.117
-
 coniferyl alcohol
-
 Sorghum bicolor
0.117
-
 coniferyl alcohol pH 8.8, temperature not specified in the publication Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-
Sorghum bicolor A1ILL4 isoform CAD2
-
Sorghum bicolor C5XC49
-
-
Sorghum bicolor C5XC49 isoform CAD4
-

Purification (Commentary)

Purification (Comment) Organism
by affinity purification on a nickel column Sorghum bicolor
recombinant enzyme Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
internode Bmr6 expression in young internodes is significantly higher than CAD4 for all genotypes Sorghum bicolor
-
internode Bmr6 expression in young internodes is significantly higher than CAD4 for all genotypes, but only 4fold to 5fold higher in bmr6 and bmr6 bmr12 internodes. Bmr6 expression is significantly decreased in bmr6 and bmr6 bmr12 compared to the wild-type and bmr12, 20fold and 15fold, respectively Sorghum bicolor
-
additional information polymorphic restriction site is present in the near-isogenic lines Atlas bmr6, RTx430 bmr6, and Wheatland bmr6 and is absent from wild-type Atlas, RTx430, and Wheatland Sorghum bicolor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl alcohol + NADP+ CAD4 displays slight activity for benzoyl alcohol Sorghum bicolor benzaldehyde + NADPH + H+
-
 ?
benzyl alcohol + NADP+ slight activity Sorghum bicolor benzylaldehyde + NADPH + H+
-
 r
coniferyl alcohol + NADP+
-
 Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 r
coniferyl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4. Activity of Bmr6 is 2.5- and 34fold higher than CAD4 for coniferyl and sinapyl aldehyde, respectively Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 ?
coniferyl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4. Bmr6 activity is 2.2- and 2.6-fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates. Activity of Bmr6 is 2.5- and 34fold higher than CAD4 for coniferyl and sinapyl aldehyde, respectively Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 ?
coniferyl alcohol + NADP+ Bmr6 activity is 2.2- and 2.6fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates compared to coniferyl alcohol as a substrate Sorghum bicolor coniferyl aldehyde + NADPH + H+
-
 r
coniferyl aldehyde + NADPH + H+ best substrate Sorghum bicolor coniferyl alcohol + NADP+
-
 r
coniferyl aldehyde + NADPH + H+ Brm6 has higher activities for the coniferyl and sinapyl aldehydes in comparison to the corresponding alcohols Sorghum bicolor coniferyl alcohol + NADP+
-
 r
coumaryl alcohol + NADP+
-
 Sorghum bicolor coumaryl aldehyde + NADPH + H+
-
 r
coumaryl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4 Sorghum bicolor coumaryl aldehyde + NADPH + H+
-
 ?
coumaryl alcohol + NADP+ Bmr6 activity is 2.2- and 2.6fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates compared to coniferyl alcohol as a substrate Sorghum bicolor coumaryl aldehyde + NADPH + H+
-
 r
additional information a C-to-T transition mutation is present in bmr6 but not in any wild-type sequence. This mutation changes amino acid 132 of the protein from Gln (CAG) to a stop codon (UAG). The bmr6 sequence encodes a predicted truncated protein that lacks the nucleotide binding (NADPH) and C-terminal catalytic domains, thus bmr6 is presumably a null allele. No activity with caffeoyl alcohol and benzoyl alcohol Sorghum bicolor ?
-
 ?
additional information CAD4 expression is significantly increased in bmr6 and bmr6 bmr12 plants relative to the wild-type and bmr12, 5- and 2.5fold, respectively, which may highlight a compensatory mechanism for loss of CAD activity in bmr6. No activity with caffeoyl alcohol Sorghum bicolor ?
-
 ?
sinapyl alcohol + NADP+
-
 Sorghum bicolor sinapyl aldehyde + NADPH + H+
-
 r
sinapyl alcohol + NADP+ Bmr6 displays significantly greater activity in comparison to CAD4 Sorghum bicolor sinapyl aldehyde + NADPH + H+
-
 ?
sinapyl alcohol + NADP+ Bmr6 activity is 2.2- and 2.6fold greater respectively, when coumaryl and sinapyl alcohols are used as substrates compared to coniferyl alcohol as a substrate Sorghum bicolor sinapyl aldehyde + NADPH + H+
-
 r
sinapyl aldehyde + NADPH + H+
-
 Sorghum bicolor sinapyl alcohol + NADP+
-
 r
sinapyl aldehyde + NADPH + H+ Brm6 has higher activities for the coniferyl and sinapyl aldehydes in comparison to the corresponding alcohols Sorghum bicolor sinapyl alcohol + NADP+
-
 r

Synonyms

Synonyms Comment Organism
Bmr6
-
 Sorghum bicolor
brown midrib6
-
 Sorghum bicolor
CAD2
-
 Sorghum bicolor
CAD4
-
 Sorghum bicolor
cinnamyl alcohol dehydrogenase
-
 Sorghum bicolor