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Literature summary for 1.1.1.195 extracted from
- Purification and properties of cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase from poplar stems (Populus X euramericana) (1984), Eur. J. Biochem., 139, 259-265.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Populus x canadensis |
| 60% loss of activity after 20 h incubation in buffer containing a divalent cation-exchange resin | Populus x canadensis |
| dithiothreitol stabilizes | Populus x canadensis |
| ethylene glycol stabilizes | Populus x canadensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00077 | - |
coniferaldehyde | - |
Populus x canadensis |  |
| 0.0012 | - |
p-coumaraldehyde | - |
Populus x canadensis | |
| 0.0048 | - |
sinapaldehyde | - |
Populus x canadensis | |
| 0.008 | - |
NADPH | - |
Populus x canadensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | restores activity after incubation in buffer containing a divalent-cation exchange resin | Populus x canadensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Populus x canadensis |
| 70000 | - |
gel filtration | Populus x canadensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus x canadensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Populus x canadensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.71 | - |
- |
Populus x canadensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, mercaptoethanol, ethylene glycol, 1 month | Populus x canadensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl alcohol + NADP+ | - |
Populus x canadensis | coniferyl aldehyde + NADPH + H+ | - |
? | |
| p-coumaryl alcohol + NADP+ | - |
Populus x canadensis | p-coumaraldehyde + NADPH | - |
? | |
| sinapyl alcohol + NADP+ | - |
Populus x canadensis | sinapaldehyde + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Populus x canadensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
reduction of coniferyl aldehyde, KH2PO4/Na2HPO4 buffer | Populus x canadensis |
| 7 | - |
reduction of coniferyl aldehyde, Tris maleate/NaOH buffer | Populus x canadensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
4°C, 1 week, 50% loss of activity | Populus x canadensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Populus x canadensis | |
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Release 2023.1 (January 2023)
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