BRENDA - Enzyme Database
show all sequences of 1.1.1.193

Identification and characterization of two Streptomyces davawensis riboflavin biosynthesis gene clusters

Grill, S.; Yamaguchi, H.; Wagner, H.; Zwahlen, L.; Kusch, U.; Mack, M.; Arch. Microbiol. 188, 377-387 (2007) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene ribG, ribG forms an expression unit with ribY, a monofunctional GTP cyclohydrolase II, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
Streptomyces davaonensis
Engineering
Protein Variants
Commentary
Organism
additional information
a ribG mutant can be rescued by expression of ribB, overview
Streptomyces davaonensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davaonensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davaonensis
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davaonensis 768
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
additional information
Streptomyces davaonensis
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
?
additional information
Streptomyces davaonensis 768
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces davaonensis
Q2VCF5
bifunctional enzyme; gene ribG
-
Streptomyces davaonensis 768
Q2VCF5
bifunctional enzyme; gene ribG
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davaonensis
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davaonensis 768
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davaonensis
?
-
-
-
?
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davaonensis 768
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davaonensis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ribG, ribG forms an expression unit with ribY, a monofunctional GTP cyclohydrolase II, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
Streptomyces davaonensis
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
a ribG mutant can be rescued by expression of ribB, overview
Streptomyces davaonensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davaonensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davaonensis
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davaonensis 768
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
additional information
Streptomyces davaonensis
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
?
additional information
Streptomyces davaonensis 768
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davaonensis
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davaonensis 768
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davaonensis
?
-
-
-
?
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davaonensis 768
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davaonensis
Other publictions for EC 1.1.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
760492
Chen
Evolution of archaeal Rib7 an ...
Bacillus subtilis, Methanosarcina mazei
Biochem. Biophys. Res. Commun.
503
195-201
2018
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-
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-
12
-
-
-
-
-
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-
2
-
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-
-
-
2
-
-
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-
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-
-
1
-
-
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-
-
-
1
-
12
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
723878
Dawson
Structure of diaminohydroxypho ...
Acinetobacter baumannii
Acta Crystallogr. Sect. F
69
611-617
2013
-
-
1
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684754
Grill
Identification and characteriz ...
Streptomyces davaonensis, Streptomyces davaonensis 768
Arch. Microbiol.
188
377-387
2007
-
-
1
-
1
-
-
-
-
-
1
4
-
6
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
1
-
-
-
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1
4
-
-
-
-
-
-
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-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246735
Richter
Biosynthesis of riboflavin: ch ...
Bacillus subtilis, Escherichia coli
J. Bacteriol.
179
2022-2028
1997
-
-
2
-
-
-
-
-
-
2
-
2
-
2
-
-
2
-
-
-
-
-
2
2
2
2
-
-
-
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
2
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
246736
Burrows
Presence in Escherichia coli o ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Bacteriol.
136
657-667
1978
-
-
-
-
-
-
-
1
-
-
1
2
-
177
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
1
-
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2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
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-