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Literature summary for 1.1.1.188 extracted from

  • Nagata, N.; Kusakari, Y.; Fukunishi, Y.; Inoue, T.; Urade, Y.
    Catalytic mechanism of the primary human prostaglandin F2alpha synthase, aldo-keto reductase 1B1 - prostaglandin D2 synthase activity in the absence of NADP(H) (2011), FEBS J., 278, 1288-1298.
    View publication on PubMed
Search for more literature for 1.1.1.188

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D43E only trace activities in presence and absence of NADPH Homo sapiens
D43N only trace activities in presence and absence of NADPH Homo sapiens
H110A only trace activities in presence and absence of NADPH Homo sapiens
H110F only trace activities in presence and absence of NADPH Homo sapiens
K77L only trace activities in presence and absence of NADPH Homo sapiens
K77R only trace activities in presence and absence of NADPH Homo sapiens
Y48F 69% of wild-type activity in presence of NADPH, 138% of wild-type isomerization activity in absence of NADPH, 0.2% of wild-type activity reducing 4-nitrobenzaldehyde Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 prostaglandin H2 pH 5.5, 37°C Homo sapiens
0.033
-
 prostaglandin H2 pH 5.0, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens P15121
-
-
Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
 pH 5.5, 37°C Homo sapiens
3.6
-
 pH 5.0, 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrobenzaldehyde + NADPH + H+
-
 Mus musculus (4-nitrophenyl)methanol + NADP+
-
 ?
4-nitrobenzaldehyde + NADPH + H+
-
 Homo sapiens (4-nitrophenyl)methanol + NADP+
-
 ?
additional information in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.018 mM. This activity activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively Mus musculus ?
-
 ?
additional information in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.023 mM. This activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively Homo sapiens ?
-
 ?
prostaglandin H2 + NADPH + H+
-
 Mus musculus 9alpha,11beta-prostaglandin F2 + NADP+
-
 ?
prostaglandin H2 + NADPH + H+
-
 Homo sapiens 9alpha,11beta-prostaglandin F2 + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Mus musculus
5.5
-
-
 Homo sapiens