BRENDA - Enzyme Database show
show all sequences of 1.1.1.187

Defects in D-rhamnosyl residue biosynthetic genes affect lipopolysaccharide structure, motility, and cell-surface hydrophobicity in Pseudomonas syringae pathovar glycinea race 4

Chiku, K.; Tsunemi, K.; Yamamoto, M.; Ohnishi-Kameyama, M.; Yoshida, M.; Ishii, T.; Taguchi, F.; Iwaki, M.; Ichinose, Y.; Ono, H.; Biosci. Biotechnol. Biochem. 77, 505-510 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene rmd
Pseudomonas syringae
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of enzyme deletion mutant DELTArmd. The mutant strain shows highly reduced antimicrobial susceptibility with chloramphenicol, ampicillin or spectinomycin. Structural analysis of lipopolysaccharides in the mutant strain, NMR and mass spectrrometry analysis, overview
Pseudomonas syringae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas syringae
-
pathovar glycinea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
-
740178
Pseudomonas syringae
GDP-alpha-D-rhamnose + NAD(P)+
-
-
-
r
Cloned(Commentary) (protein specific)
Commentary
Organism
gene rmd
Pseudomonas syringae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of enzyme deletion mutant DELTArmd. The mutant strain shows highly reduced antimicrobial susceptibility with chloramphenicol, ampicillin or spectinomycin. Structural analysis of lipopolysaccharides in the mutant strain, NMR and mass spectrrometry analysis, overview
Pseudomonas syringae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
-
740178
Pseudomonas syringae
GDP-alpha-D-rhamnose + NAD(P)+
-
-
-
r
General Information
General Information
Commentary
Organism
malfunction
effect of deficiency in GDP-D-rhamnose biosynthetic genes, including GDP-4-keto-6-deoxy-D-mannose reductase, on lipopolysaccharide structure and pathogenicity in soybean, Glycine max cv. Shirofumi. The mutant strain DELTArmd shows highly reduced antimicrobial susceptibility with chloramphenicol, ampicillinm or spectinomycin. The mutant DELTArmd strain also shows increased degrees of adhesion to the polystyrene surface of the micro-titrate plates. It has higher hydrophobicity of the cell surface than the wild-type strain, but has normal flagellin glycans
Pseudomonas syringae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
effect of deficiency in GDP-D-rhamnose biosynthetic genes, including GDP-4-keto-6-deoxy-D-mannose reductase, on lipopolysaccharide structure and pathogenicity in soybean, Glycine max cv. Shirofumi. The mutant strain DELTArmd shows highly reduced antimicrobial susceptibility with chloramphenicol, ampicillinm or spectinomycin. The mutant DELTArmd strain also shows increased degrees of adhesion to the polystyrene surface of the micro-titrate plates. It has higher hydrophobicity of the cell surface than the wild-type strain, but has normal flagellin glycans
Pseudomonas syringae
Other publictions for EC 1.1.1.187
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740178
Chiku
Defects in D-rhamnosyl residue ...
Pseudomonas syringae
Biosci. Biotechnol. Biochem.
77
505-510
2013
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1
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651142
Suzuki
Guanosine diphosphate-4-keto-6 ...
Aggregatibacter actinomycetemcomitans, Aggregatibacter actinomycetemcomitans SUNYaB 75
Eur. J. Biochem.
269
5963-5971
2002
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1
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389366
Winkler
Guanosine diphosphate-4-keto-D ...
Pseudomonas sp.
J. Biol. Chem.
246
5868-5876
1971
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1
4
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1
2
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1
1
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2
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1
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4
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2
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1
1
2
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1
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2
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389367
Barber
The synthesis of guanosine 5-d ...
Leucaena leucocephala
Biochim. Biophys. Acta
165
68-75
1968
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1
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