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Literature summary for 1.1.1.184 extracted from

  • Breysse, D.H.; Boone, R.M.; Long, C.M.; Merrill, M.E.; Schaupp, C.M.; White, C.C.; Kavanagh, T.J.; Schmidt, E.E.; Merrill, G.F.
    Carbonyl reductase 1 plays a significant role in converting doxorubicin to cardiotoxic doxorubicinol in mouse liver, but the majority of the doxorubicinol-forming activity remains unidentified (2020), Drug Metab. Dispos., 48, 187-197 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 doxorubicin isoform Cbr3, at pH 7.0 and 37°C Mus musculus
0.074
-
 NADPH isoform Cbr3, at pH 7.0 and 37°C Mus musculus
0.099
-
 NADPH isoform Cbr1, at pH 7.0 and 37°C Mus musculus
0.13
-
 doxorubicin isoform Cbr1, at pH 7.0 and 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Organism

Organism UniProt Comment Textmining
Mus musculus P48758
-
-
Mus musculus Q8K354
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
doxorubicin + NADPH + H+
-
 Mus musculus doxorubicinol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
carbonyl reductase 1
-
 Mus musculus
carbonyl reductase 3
-
 Mus musculus
CBR1
-
 Mus musculus
CBR3
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0001
-
 NADPH isoform Cbr3, at pH 7.0 and 37°C Mus musculus
0.0014
-
 doxorubicin isoform Cbr3, at pH 7.0 and 37°C Mus musculus
0.015
-
 doxorubicin isoform Cbr1, at pH 7.0 and 37°C Mus musculus
0.036
-
 NADPH isoform Cbr1, at pH 7.0 and 37°C Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0013
-
 NADPH isoform Cbr3, at pH 7.0 and 37°C Mus musculus
0.035
-
 doxorubicin isoform Cbr3, at pH 7.0 and 37°C Mus musculus
0.12
-
 doxorubicin isoform Cbr1, at pH 7.0 and 37°C Mus musculus
0.36
-
 NADPH isoform Cbr1, at pH 7.0 and 37°C Mus musculus