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Literature summary for 1.1.1.184 extracted from

  • Naeem, M.; Rehman, A.; Shen, B.; Ye, L.; Yu, H.
    Semi-rational engineering of carbonyl reductase YueD for efficient biosynthesis of halogenated alcohols with in situ cofactor regeneration (2018), Biochem. Eng. J., 137, 62-70 .
No PubMed abstract available
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
G135A the mutant shows decreased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
G180A the mutant shows slightly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
I190A the mutant shows decreased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
M186A the mutant shows increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
Q187A the mutant shows increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
V181A the mutant shows strongly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
W144A the mutant shows slightly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis
Y141A the mutant shows slightly increased activity with 3-bromoacetophenone as compared to the wild type enzyme Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis WB600
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(3-nitrosophenyl)ethan-1-one + NADPH + H+ 86% conversion after 24 h, 99% R-enantiomeric excess Bacillus subtilis 1-(3-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1-(3-nitrosophenyl)ethan-1-one + NADPH + H+ 86% conversion after 24 h, 99% R-enantiomeric excess Bacillus subtilis WB600 1-(3-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1-(4-chlorophenyl)ethan-1-one + NADPH + H+ 41% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis 1-(4-chlorophenyl)ethan-1-ol + NADP+
-
 ?
1-(4-chlorophenyl)ethan-1-one + NADPH + H+ 41% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis WB600 1-(4-chlorophenyl)ethan-1-ol + NADP+
-
 ?
1-(4-nitrosophenyl)ethan-1-one + NADPH + H+ 30% conversion after 24 h, 73% S-enantiomeric excess Bacillus subtilis 1-(4-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1-(4-nitrosophenyl)ethan-1-one + NADPH + H+ 30% conversion after 24 h, 73% S-enantiomeric excess Bacillus subtilis WB600 1-(4-nitrosophenyl)ethan-1-ol + NADP+
-
 ?
1-phenylethan-1-one + NADPH + H+ 75% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis 1-phenylethan-1-ol + NADP+
-
 ?
1-phenylethan-1-one + NADPH + H+ 75% conversion after 24 h, 99% S-enantiomeric excess Bacillus subtilis WB600 1-phenylethan-1-ol + NADP+
-
 ?
2,2,2-trifluoro-1-phenylethan-1-one + NADPH + H+ 99.6% conversion after 24 h, 91.5% R-enantiomeric excess Bacillus subtilis 2,2,2-trifluoro-1-phenylethan-1-ol + NADP+
-
 ?
2,2,2-trifluoro-1-phenylethan-1-one + NADPH + H+ 99.6% conversion after 24 h, 91.5% R-enantiomeric excess Bacillus subtilis WB600 2,2,2-trifluoro-1-phenylethan-1-ol + NADP+
-
 ?
2-chloro-1-(3,4-dichlorophenyl)ethan-1-one + NADPH + H+ 41% conversion after 24 h, 99% R-enantiomeric excess Bacillus subtilis 2-chloro-1-(3,4-dichlorophenyl)ethan-1-ol + NADP+
-
 ?
3-bromoacetophenone + NADPH + H+ 61% conversion after 24 h Bacillus subtilis 1-(3-bromophenyl)ethanol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
YueD
-
 Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30 more than 60% activity between 20 and 30°C Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6 8 more than 50% activity between pH 6.0 and 8.0. The activity decreases rapidly when pH is below 6.0 or above 8.0 Bacillus subtilis