Application | Comment | Organism |
---|---|---|
synthesis | in situ expression in Candida leads to over fourfold higher activity for conversion of racemic (R,S)-1-phenyl-1,2-ethanediol to 2-hydroxyacetophenone, while maintaining the activity for catalyzing 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol. A recombinant Candida parapsilosis converts racemic (R,S)-1-phenyl-1,2-ethanediol to its (S)-isomer with an optical purity of 98.8% and a yield of 48.4%. The biotransformation duration is reduced from 48 to 13 h | Candida parapsilosis CCTCC M203011 |
Cloned (Comment) | Organism |
---|---|
- |
Candida parapsilosis CCTCC M203011 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis CCTCC M203011 | A1X808 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-phenyl-1,2-ethanediol + NADH + H+ | - |
Candida parapsilosis CCTCC M203011 | 2-hydroxyacetophenone + NAD+ | - |
r | |
hydroxyacetophenone + NAD+ | - |
Candida parapsilosis CCTCC M203011 | (R)-1-phenyl-1,2-ethanediol + NADH + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
oxidation of (R)-1-phenyl-1,2-ethanediol | Candida parapsilosis CCTCC M203011 |
35 | - |
reduction of hydroxyacetophenone | Candida parapsilosis CCTCC M203011 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
oxidation of (R)-1-phenyl-1,2-ethanediol | Candida parapsilosis CCTCC M203011 |
6.5 | - |
reduction of hydroxyacetophenone | Candida parapsilosis CCTCC M203011 |