Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Kluyveromyces marxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
0.034 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
0.43 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.6 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35800 | - |
gel filtration | Kluyveromyces marxianus |
36900 | - |
gel filtration | Kluyveromyces marxianus |
37000 | - |
1 * 37000, SDS-PAGE | Kluyveromyces marxianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | A0A0E4AX59 | - |
- |
Kluyveromyces marxianus | A0A0E4AY21 | - |
- |
Kluyveromyces marxianus DMB1 | A0A0E4AX59 | - |
- |
Kluyveromyces marxianus DMB1 | A0A0E4AY21 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.58 | - |
substrate benzaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
0.6 | - |
substrate benzaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
0.65 | - |
substrate furfural, pH 6.0, 25°C | Kluyveromyces marxianus |
0.96 | - |
substrate methyl glyoxal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1 | - |
substrate p-anisaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.2 | - |
substrate furfural, pH 6.0, 25°C | Kluyveromyces marxianus |
1.5 | - |
substrate hexanal, pH 6.0, 25°C | Kluyveromyces marxianus |
2 | - |
substrate hexanal, pH 6.0, 25°C | Kluyveromyces marxianus |
2.1 | - |
substrate octanal, pH 6.0, 25°C | Kluyveromyces marxianus |
2.1 | - |
substrate valeraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
3 | - |
substrate heptanal, pH 6.0, 25°C | Kluyveromyces marxianus |
3.2 | - |
substrate methyl glyoxal, pH 6.0, 25°C | Kluyveromyces marxianus |
3.3 | - |
substrate isovaleraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
4.2 | - |
substrate isovaleraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
4.5 | - |
substrate p-anisaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
5.3 | - |
substrate octanal, pH 6.0, 25°C | Kluyveromyces marxianus |
5.9 | - |
substrate valeraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
6.9 | - |
substrate heptanal, pH 6.0, 25°C | Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | benzyl alcohol + NADP+ | - |
? | |
benzaldehyde + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | benzyl alcohol + NADP+ | - |
? | |
furfural + NADPH + H+ | - |
Kluyveromyces marxianus | furfuryl alcohol + NADP+ | - |
? | |
heptanal + NADPH + H+ | - |
Kluyveromyces marxianus | heptan-1-ol + NADP+ | - |
? | |
heptanal + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | heptan-1-ol + NADP+ | - |
? | |
hexanal + NADPH + H+ | - |
Kluyveromyces marxianus | hexan-1-ol + NADP+ | - |
? | |
isovaleraldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | isoamyl alcohol + NADP+ | - |
? | |
isovaleraldehyde + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | isoamyl alcohol + NADP+ | - |
? | |
methyl glyoxal + NADPH + H+ | - |
Kluyveromyces marxianus | ? + NADP+ | - |
? | |
methyl glyoxal + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | ? + NADP+ | - |
? | |
additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward 5-hydroxymethylfurfural, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus | ? | - |
? | |
additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus | ? | - |
? | |
additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward 5-hydroxymethylfurfural, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus DMB1 | ? | - |
? | |
additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus DMB1 | ? | - |
? | |
octanal + NADPH + H+ | - |
Kluyveromyces marxianus | octan-1-ol + NADP+ | - |
? | |
p-anisaldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | p-anisalcohol + NADP+ | - |
? | |
valeraldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | pentan-1-ol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Kluyveromyces marxianus |
Synonyms | Comment | Organism |
---|---|---|
YGL039w1 | - |
Kluyveromyces marxianus |
YGL039w2 | - |
Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | - |
Kluyveromyces marxianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, loss of more than 50% of activity | Kluyveromyces marxianus |
47 | - |
melting temperature | Kluyveromyces marxianus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus | |
2.67 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
3.67 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
4 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Kluyveromyces marxianus | |
NADPH | exclusive use of NADPH | Kluyveromyces marxianus |