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Literature summary for 1.1.1.184 extracted from

  • Fukuda, Y.; Sone, T.; Sakuraba, H.; Araki, T.; Ohshima, T.; Shibata, T.; Yoneda, K.
    A novel NAD(P)H-dependent carbonyl reductase specifically expressed in the thyroidectomized chicken fatty liver: catalytic properties and crystal structure (2015), FEBS J., 282, 3918-3928.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gallus gallus

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular replacement, to 1.98 A resolution in presence of NADPH and ethylene glycol Gallus gallus

Protein Variants

Protein Variants Comment Organism
Y172A Km value for 9,10-phenanthrenequinone (model substrate) is 12.5 times higher than that for the wild-type enzyme Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
estrone pH 6.5, 25°C Gallus gallus
0.002
-
9,10-phenanthrenequinone pH 6.5, 25°C Gallus gallus
0.03
-
prostaglandin E2 pH 6.5, 25°C Gallus gallus
0.04
-
menadione pH 6.5, 25°C Gallus gallus
0.04
-
testosterone pH 6.5, 25°C Gallus gallus
0.04
-
isatin pH 6.5, 25°C Gallus gallus
0.05
-
acetone pH 6.5, 25°C Gallus gallus
0.18
-
p-toluquinone pH 6.5, 25°C Gallus gallus
0.44
-
4-nitrobenzaldehyde pH 6.5, 25°C Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus F1N9C1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrobenzaldehyde + NADPH + H+
-
Gallus gallus 4-nitrobenzyl alcohol + NADP+
-
?
9,10-phenanthrenequinone + NADPH + H+
-
Gallus gallus ? + NADP+
-
?
acetone + NADPH + H+
-
Gallus gallus 2-propanol + NADP+
-
?
estrone + NADPH + H+
-
Gallus gallus estradiol + NADP+
-
?
isatin + NADPH + H+
-
Gallus gallus 3-hydroxy-2-oxoindole + NADP+
-
?
menadione + NADPH + H+
-
Gallus gallus ? + NADP+
-
?
p-toluquinone + NADPH + H+
-
Gallus gallus ? + NADP+
-
?
prostaglandin E2 + NADPH + H+
-
Gallus gallus prostaglandin F2alpha + NADP+
-
?
testosterone + NADPH + H+
-
Gallus gallus ?
-
?

Synonyms

Synonyms Comment Organism
LOC415661 locus name Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, 50% residual activity Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
acetone pH 6.5, 25°C Gallus gallus
0.27
-
menadione pH 6.5, 25°C Gallus gallus
0.45
-
estrone pH 6.5, 25°C Gallus gallus
0.6
-
testosterone pH 6.5, 25°C Gallus gallus
0.68
-
prostaglandin E2 pH 6.5, 25°C Gallus gallus
2.33
-
9,10-phenanthrenequinone pH 6.5, 25°C Gallus gallus
2.55
-
isatin pH 6.5, 25°C Gallus gallus
3.28
-
p-toluquinone pH 6.5, 25°C Gallus gallus
3.48
-
4-nitrobenzaldehyde pH 6.5, 25°C Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
-
Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 10 min, 40°C, at least 50% residual activity Gallus gallus