Cloned (Comment) | Organism |
---|---|
mutants overexpressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | residues of CBR3 exchanged to the corresponding amino acids of CBR1. Mutation of nine residues (236-244) in the vicinity of the catalytic center and a proline (P230) in CBR3 to the corresponding residues of CBR1 reveals a much higher kcat/Km value (5.7 micromol/min) towards isatin | Homo sapiens |
P230W | active towards isatin and 9,10-phenanthrenequinone as the wild-type | Homo sapiens |
V244M | inactive towards menadione, active towards isatin and 9,10-phenanthrenequinone as the wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
9,10-phenanthrenequinone | residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0078 | - |
isatin | wild-type CBR1 | Homo sapiens | |
0.0089 | - |
9,10-phenanthrenequinone | residues 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0092 | - |
9,10-phenanthrenequinone | residues 97-98, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0225 | - |
isatin | residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0315 | - |
isatin | residues 142-143, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0327 | - |
isatin | residues 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0354 | - |
9,10-phenanthrenequinone | wild-type CBR1 | Homo sapiens | |
0.0393 | - |
9,10-phenanthrenequinone | residues 142-143, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0471 | - |
isatin | residues 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.055 | - |
isatin | residues 97-98, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.0564 | - |
9,10-phenanthrenequinone | residues 230, 236244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
0.078 | - |
9,10-phenanthrenequinone | wild-type CBR3 and mutant V244M. Residues 236-244, 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
1.6 | - |
isatin | residues 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens | |
4 | - |
isatin | wild-type CBR3 and mutant V244M. Residues 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75828 | - |
- |
Homo sapiens | P16152 | - |
- |
Purification (Comment) | Organism |
---|---|
mutants purfied by Ni-affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9,10-phenanthrenequinone + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
isatin + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
additional information | inactive towards menadione | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Homo sapiens |
CBR1 | - |
Homo sapiens |
CBR3 | - |
Homo sapiens |