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Literature summary for 1.1.1.184 extracted from

  • Miura, T.; Itoh, Y.; Takada, M.; Tsutsui, H.; Yukimura, T.; Nishinaka, T.; Terada, T.
    Investigation of the role of the amino acid residue at position 230 for catalysis in monomeric carbonyl reductase 3 (2009), Chem. Biol. Interact., 178, 211-214.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells Cricetulus griseus
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells Homo sapiens
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
P230W exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates Homo sapiens
W230P exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates Cricetulus griseus
W230P exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000000053
-
4-benzoylpyridine wild-type Homo sapiens
0.000000058
-
4-benzoylpyridine mutant P230W Homo sapiens
0.000000067
-
menadione wild-type Homo sapiens
0.000000079
-
menadione mutant P230W Homo sapiens
0.000000089
-
menadione wild-type Cricetulus griseus
0.000000097
-
menadione mutant W230P Cricetulus griseus
0.0000003
-
menadione wild-type and mutant W230P Rattus norvegicus
0.00000054
-
4-benzoylpyridine mutant W230P Cricetulus griseus
0.00000062
-
4-benzoylpyridine wild-type Cricetulus griseus
0.0000015
-
4-benzoylpyridine wild-type Rattus norvegicus
0.0000018
-
4-benzoylpyridine mutant W230P Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus B2GV72 Sprague-Dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+ chelating affinity column chromatography Cricetulus griseus
by Ni2+ chelating affinity column chromatography Homo sapiens
by Ni2+ chelating affinity column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-benzoylpyridine + NADPH + H+
-
Cricetulus griseus (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
?
4-benzoylpyridine + NADPH + H+
-
Homo sapiens (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
?
4-benzoylpyridine + NADPH + H+
-
Rattus norvegicus (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
?
5alpha-androstane-17beta-ol-3-one + NADPH
-
Cricetulus griseus ? + NADP+
-
?
5alpha-androstane-17beta-ol-3-one + NADPH
-
Homo sapiens ? + NADP+
-
?
5alpha-androstane-17beta-ol-3-one + NADPH
-
Rattus norvegicus ? + NADP+
-
?
5alpha-androstane-3,17-dione + NADPH
-
Cricetulus griseus ? + NADP+
-
?
5alpha-androstane-3,17-dione + NADPH
-
Homo sapiens ? + NADP+
-
?
5alpha-androstane-3,17-dione + NADPH
-
Rattus norvegicus ? + NADP+
-
?
5beta-pregnan-3alpha-ol-20-one + NADPH
-
Cricetulus griseus ? + NADP+
-
?
5beta-pregnan-3alpha-ol-20-one + NADPH
-
Homo sapiens ? + NADP+
-
?
menadione + NADPH
-
Cricetulus griseus ? + NADP+
-
?
menadione + NADPH
-
Homo sapiens ? + NADP+
-
?
menadione + NADPH
-
Rattus norvegicus ? + NADP+
-
?
additional information no activity with 5alpha-androstane-3alpha-ol-17-one Cricetulus griseus ?
-
?
additional information no activity with 5alpha-androstane-3alpha-ol-17-one Homo sapiens ?
-
?
additional information no activity with 5alpha-androstane-3alpha-ol-17-one Rattus norvegicus ?
-
?
additional information no activity with 5beta-pregnan-3alpha-ol-20-one Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Cricetulus griseus
monomer
-
Homo sapiens
monomer
-
Rattus norvegicus

Synonyms

Synonyms Comment Organism
carbonyl reductase 3
-
Cricetulus griseus
carbonyl reductase 3
-
Homo sapiens
carbonyl reductase 3
-
Rattus norvegicus
CBR3
-
Cricetulus griseus
CBR3
-
Homo sapiens
CBR3
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009167
-
4-benzoylpyridine mutant P230W Homo sapiens
0.0103
-
4-benzoylpyridine wild-type Homo sapiens
0.011
-
menadione wild-type Homo sapiens
0.01217
-
menadione mutant P230W Homo sapiens
0.04167
-
menadione mutant W230P Cricetulus griseus
0.0483
-
menadione wild-type Cricetulus griseus
0.063
-
4-benzoylpyridine wild-type Cricetulus griseus
0.065
-
4-benzoylpyridine mutant W230P Cricetulus griseus
0.183
-
4-benzoylpyridine mutant W230P Rattus norvegicus
0.23
-
4-benzoylpyridine wild-type Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Cricetulus griseus
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus