Cloned (Comment) | Organism |
---|---|
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells | Cricetulus griseus |
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells | Homo sapiens |
wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
P230W | exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates | Homo sapiens |
W230P | exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates | Cricetulus griseus |
W230P | exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000053 | - |
4-benzoylpyridine | wild-type | Homo sapiens | |
0.000000058 | - |
4-benzoylpyridine | mutant P230W | Homo sapiens | |
0.000000067 | - |
menadione | wild-type | Homo sapiens | |
0.000000079 | - |
menadione | mutant P230W | Homo sapiens | |
0.000000089 | - |
menadione | wild-type | Cricetulus griseus | |
0.000000097 | - |
menadione | mutant W230P | Cricetulus griseus | |
0.0000003 | - |
menadione | wild-type and mutant W230P | Rattus norvegicus | |
0.00000054 | - |
4-benzoylpyridine | mutant W230P | Cricetulus griseus | |
0.00000062 | - |
4-benzoylpyridine | wild-type | Cricetulus griseus | |
0.0000015 | - |
4-benzoylpyridine | wild-type | Rattus norvegicus | |
0.0000018 | - |
4-benzoylpyridine | mutant W230P | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | B2GV72 | Sprague-Dawley rats | - |
Purification (Comment) | Organism |
---|---|
by Ni2+ chelating affinity column chromatography | Cricetulus griseus |
by Ni2+ chelating affinity column chromatography | Homo sapiens |
by Ni2+ chelating affinity column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-benzoylpyridine + NADPH + H+ | - |
Cricetulus griseus | (S)-alpha-phenyl-4-pyridylmethanol + NADP+ | - |
? | |
4-benzoylpyridine + NADPH + H+ | - |
Homo sapiens | (S)-alpha-phenyl-4-pyridylmethanol + NADP+ | - |
? | |
4-benzoylpyridine + NADPH + H+ | - |
Rattus norvegicus | (S)-alpha-phenyl-4-pyridylmethanol + NADP+ | - |
? | |
5alpha-androstane-17beta-ol-3-one + NADPH | - |
Cricetulus griseus | ? + NADP+ | - |
? | |
5alpha-androstane-17beta-ol-3-one + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
5alpha-androstane-17beta-ol-3-one + NADPH | - |
Rattus norvegicus | ? + NADP+ | - |
? | |
5alpha-androstane-3,17-dione + NADPH | - |
Cricetulus griseus | ? + NADP+ | - |
? | |
5alpha-androstane-3,17-dione + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
5alpha-androstane-3,17-dione + NADPH | - |
Rattus norvegicus | ? + NADP+ | - |
? | |
5beta-pregnan-3alpha-ol-20-one + NADPH | - |
Cricetulus griseus | ? + NADP+ | - |
? | |
5beta-pregnan-3alpha-ol-20-one + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
menadione + NADPH | - |
Cricetulus griseus | ? + NADP+ | - |
? | |
menadione + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
menadione + NADPH | - |
Rattus norvegicus | ? + NADP+ | - |
? | |
additional information | no activity with 5alpha-androstane-3alpha-ol-17-one | Cricetulus griseus | ? | - |
? | |
additional information | no activity with 5alpha-androstane-3alpha-ol-17-one | Homo sapiens | ? | - |
? | |
additional information | no activity with 5alpha-androstane-3alpha-ol-17-one | Rattus norvegicus | ? | - |
? | |
additional information | no activity with 5beta-pregnan-3alpha-ol-20-one | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Cricetulus griseus |
monomer | - |
Homo sapiens |
monomer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase 3 | - |
Cricetulus griseus |
carbonyl reductase 3 | - |
Homo sapiens |
carbonyl reductase 3 | - |
Rattus norvegicus |
CBR3 | - |
Cricetulus griseus |
CBR3 | - |
Homo sapiens |
CBR3 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009167 | - |
4-benzoylpyridine | mutant P230W | Homo sapiens | |
0.0103 | - |
4-benzoylpyridine | wild-type | Homo sapiens | |
0.011 | - |
menadione | wild-type | Homo sapiens | |
0.01217 | - |
menadione | mutant P230W | Homo sapiens | |
0.04167 | - |
menadione | mutant W230P | Cricetulus griseus | |
0.0483 | - |
menadione | wild-type | Cricetulus griseus | |
0.063 | - |
4-benzoylpyridine | wild-type | Cricetulus griseus | |
0.065 | - |
4-benzoylpyridine | mutant W230P | Cricetulus griseus | |
0.183 | - |
4-benzoylpyridine | mutant W230P | Rattus norvegicus | |
0.23 | - |
4-benzoylpyridine | wild-type | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Cricetulus griseus | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus |