Cloned (Comment) | Organism |
---|---|
expression in Sacchaormyces cerevisiae | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
computational docking experiments for four inhibitors. For flavonoid inhibitors, the flavonoid skeleton is the binding part of the molecule and sugar moieties are pointing outward, giving rise to a stabilizing effect | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E)-1-(2,4-dihydroxyphenyl)-3-(2',4',6-trihydroxy-5'-[(2E)-3-(4-hydroxyphenyl)prop-2-enoyl]biphenyl-3-yl)prop-2-en-1-one | - |
Homo sapiens | |
3-(2-(2,4-dihydroxyphenyl)-1-[hydroxy(4-hydroxyphenyl)methyl]-2-oxoethyl)-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one | - |
Homo sapiens | |
3-[2,3-dihydroxy-4-methoxy-5-(3-methylbut-2-en-1-yl)phenyl]-5,7-dihydroxy-4H-chromen-4-one | - |
Homo sapiens | |
3-[2-(2,4-dihydroxyphenyl)-1-(4-hydroxybenzyl)-2-oxoethyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one | - |
Homo sapiens | |
7-O-beta-D-glucopyranos2-ulosyl-quercetin | - |
Homo sapiens | |
quercetin | - |
Homo sapiens | |
quercitrin | - |
Homo sapiens | |
rutin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0852 | - |
daunorubicin | pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16152 | isoform CBR1 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
161.6 | - |
substrate daunorubicin, pH 7.4, 37°C | Homo sapiens |
1533 | - |
substrate 2,3-butanedione, pH 7.4, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butanedione + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
2-hydroxy-3-butanone + NADPH | - |
Homo sapiens | 2,3-dihydroxybutanol + NADP+ | - |
? | |
daunorubicin + NADPH | - |
Homo sapiens | daunorubicinol + NADP+ | - |
? | |
doxorubicin + NADPH | - |
Homo sapiens | doxorubicinol + NADP+ | - |
? | |
ethyl acetoacetate + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
menadione + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0011 | - |
pH 7.4, 37°C | Homo sapiens | 3-[2-(2,4-dihydroxyphenyl)-1-(4-hydroxybenzyl)-2-oxoethyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one | |
0.0021 | - |
pH 7.4, 37°C | Homo sapiens | rutin | |
0.0036 | - |
pH 7.4, 37°C | Homo sapiens | 3-(2-(2,4-dihydroxyphenyl)-1-[hydroxy(4-hydroxyphenyl)methyl]-2-oxoethyl)-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one | |
0.0042 | - |
pH 7.4, 37°C | Homo sapiens | (2E)-1-(2,4-dihydroxyphenyl)-3-(2',4',6-trihydroxy-5'-[(2E)-3-(4-hydroxyphenyl)prop-2-enoyl]biphenyl-3-yl)prop-2-en-1-one | |
0.0061 | - |
pH 7.4, 37°C | Homo sapiens | 3-[2,3-dihydroxy-4-methoxy-5-(3-methylbut-2-en-1-yl)phenyl]-5,7-dihydroxy-4H-chromen-4-one | |
0.0062 | - |
pH 7.4, 37°C | Homo sapiens | quercetin | |
0.0065 | - |
pH 7.4, 37°C | Homo sapiens | quercitrin | |
0.008 | - |
pH 7.4, 37°C | Homo sapiens | 7-O-beta-D-glucopyranos2-ulosyl-quercetin |