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Literature summary for 1.1.1.184 extracted from
- Purification and characterization of a novel pyrazole-sensitive carbonyl reductase in guinea pig lung (1982), Arch. Biochem. Biophys., 217, 564-573.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 20% v/v, stabilizes | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Cavia porcellus |  |
| FeSO4 | - |
Cavia porcellus | |
| N-ethylmaleimide | - |
Cavia porcellus | |
| NAD+ | - |
Cavia porcellus | |
| NADP+ | - |
Cavia porcellus | |
| nicotinic acid | weak | Cavia porcellus | |
| p-hydroxymercuribenzoate | - |
Cavia porcellus | |
| phenylpyruvic acid | - |
Cavia porcellus | |
| pyrazole | - |
Cavia porcellus | |
| quercetin | - |
Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cavia porcellus | |
| 0.002 | - |
pyridine 4-aldehyde | cofactor NADPH | Cavia porcellus | |
| 0.004 | - |
4-Nitroacetophenone | cofactor NADPH | Cavia porcellus | |
| 0.009 | - |
menadione | cofactor NADPH | Cavia porcellus | |
| 0.012 | - |
methyl-1,4-benzoquinone | cofactor NADPH | Cavia porcellus | |
| 0.015 | - |
pyridine 3-aldehyde | cofactor NADPH | Cavia porcellus | |
| 0.031 | - |
Benzalacetone | cofactor NADPH | Cavia porcellus | |
| 0.039 | - |
acetaldehyde | cofactor NADPH | Cavia porcellus | |
| 0.057 | - |
methyl-1,4-benzoquinone | cofactor NADH | Cavia porcellus | |
| 0.091 | - |
acetone | cofactor NADPH | Cavia porcellus | |
| 0.12 | - |
crotonaldehyde | cofactor NADPH | Cavia porcellus | |
| 0.13 | - |
pyridine 3-aldehyde | cosubstrate NADH | Cavia porcellus | |
| 0.24 | - |
4-benzoylpyridine | cofactor NADPH | Cavia porcellus | |
| 0.33 | - |
Benzalacetone | cofactor NADH | Cavia porcellus | |
| 0.5 | - |
Pyridine-4-aldehyde | cofactor NADH | Cavia porcellus | |
| 3.1 | - |
acetone | cofactor NADH | Cavia porcellus | |
| 3.3 | - |
4-benzoylpyridine | cofactor NADH | Cavia porcellus | |
| 5.1 | - |
acetaldehyde | cofactor NADH | Cavia porcellus | |
| 250 | - |
crotonaldehyde | cofactor NADH | Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, SDS-PAGE | Cavia porcellus |
| 86000 | - |
gel filtration | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 enzyme species | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or 4°C, 20% v/v, glycerol, stable for 1 month | Cavia porcellus |
| 4°C, 0.02 mg/ml protein concentration, 0.15 M KCl, stable for at least several days, 90% loss of activity after 2 weeks | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-benzoylpyridine + NADPH | - |
Cavia porcellus | ? | - |
? | |
| 4-nitroacetophenone + NADPH | - |
Cavia porcellus | ? | - |
? | |
| acetaldehyde + NADPH + H+ | - |
Cavia porcellus | ethanol + NADP+ | - |
? | |
| acetone + NADPH | - |
Cavia porcellus | propan-2-ol + NADP+ | - |
? | |
| benzalacetone + NADPH | - |
Cavia porcellus | ? | - |
? | |
| crotonaldehyde + NADPH | - |
Cavia porcellus | 2-buten-1-ol + NADP+ | - |
? | |
| menadione + NADPH | - |
Cavia porcellus | ? | - |
? | |
| methyl-1,4-benzoquinone + NADPH | - |
Cavia porcellus | ? | - |
? | |
| additional information | overview | Cavia porcellus | ? | - |
? | |
| additional information | NADH also acts as cofactor | Cavia porcellus | ? | - |
? | |
| additional information | low reverse direction only detected with cyclohexanol | Cavia porcellus | ? | - |
? | |
| pyridine-3-aldehyde + NADPH | - |
Cavia porcellus | ? | - |
? | |
| pyridine-4-aldehyde + NADPH | - |
Cavia porcellus | ? | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, SDS-PAGE | Cavia porcellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 6-11, 15 min, stable | Cavia porcellus |
| 50 | - |
pH 6-11, 15 min, about 20% loss of activity | Cavia porcellus |
| 60 | - |
rapid inactivation above | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
NADPH + 4-nitroacetophenone | Cavia porcellus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
unstable | Cavia porcellus |
| 6 | 11 | 25°C, 15 min, stable | Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Cavia porcellus | |
| NADPH | - |
Cavia porcellus | |
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