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Literature summary for 1.1.1.183 extracted from

  • Hassan, M.; Maarof, N.D.; Ali, Z.M.; Noor, N.M.; Othman, R.; Mori, N.
    Monoterpene alcohol metabolism: identification, purification, and characterization of two geraniol dehydrogenase isoenzymes from Polygonum minus leaves (2012), Biosci. Biotechnol. Biochem., 76, 1463-1470.
    View publication on PubMed
Search for more literature for 1.1.1.183

Activating Compound

Activating Compound Comment Organism Structure
additional information adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity Persicaria minor

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM Persicaria minor
cyanide isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM Persicaria minor
iodoacetamide isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM Persicaria minor
MgCl2 isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM Persicaria minor
Sodium azide isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM Persicaria minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 NADP+ isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.0588
-
 NADP+ isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.185
-
 geraniol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.4
-
 geraniol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.435
-
 cinnamyl alcohol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.556
-
 cinnamyl alcohol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.714
-
 nerol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.741
-
 linalool isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
1.212
-
 nerol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
1.333
-
 linalool isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
2.222
-
 3,7-dimethyloct-6-en-1-ol isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
10
-
 3,7-dimethyloct-6-en-1-ol isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE Persicaria minor
58000
-
 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE Persicaria minor
60000
-
 1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE Persicaria minor
62000
-
 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE Persicaria minor
100000
-
 isoform geraniol-DH I, gel filtration Persicaria minor
120000
-
 isoform geraniol-DH II, gel filtration Persicaria minor

Organism

Organism UniProt Comment Textmining
Persicaria minor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography Persicaria minor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Persicaria minor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.175
-
 isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor
0.261
-
 isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C Persicaria minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-citronellol + NADP+ compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate Persicaria minor ?
-
 ?
borneol + NADP+ compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate Persicaria minor ?
-
 ?
carveol + NADP+ compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate Persicaria minor ?
-
 ?
cinnamyl alcohol + NADP+ compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate Persicaria minor ?
-
 ?
geraniol + NADP+ 100% activity Persicaria minor geranial + NADPH + H+
-
 ?
linalool + NADP+ compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate Persicaria minor ?
-
 ?
additional information tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol Persicaria minor ?
-
 ?
nerol + NADP+ compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate Persicaria minor ?
-
 ?
p-cumic alcohol + NADP+ compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate Persicaria minor ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE Persicaria minor
heterodimer 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE Persicaria minor

Synonyms

Synonyms Comment Organism
geraniol-DH I isoform Persicaria minor
geraniol-DH II isoform Persicaria minor
NADP+-dependent geraniol dehydrogenase
-
 Persicaria minor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoforms geraniol-DH I and geraniol-DH II Persicaria minor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 rapid inactiviation of isoform geraniol-DH I occurs from 30-60°C with 60% activity at 40°C and 40% residual activity at 60°C. At 70°C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40°C, while from 30-50°C the activity decreases slightly and is lost at temperatures above 50°C Persicaria minor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 isoform geraniol-DH I Persicaria minor
9.5
-
 isoform geraniol-DH II Persicaria minor

pH Range

pH Minimum pH Maximum Comment Organism
6 10 isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below Persicaria minor

pI Value

Organism Comment pI Value Maximum pI Value
Persicaria minor isoform geraniol-DH II, isoelectric focusing
-
 3.5
Persicaria minor isoform geraniol-DH I, isoelectric focusing
-
 8.8