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Literature summary for 1.1.1.183 extracted from
- Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae) (2008), FEBS J., 275, 2807-2817.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis | Carpoglyphus lactis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.051 | - |
geraniol | pH 9.0, 25°C | Carpoglyphus lactis |  |
| 0.0596 | - |
NAD+ | pH 9.0, 25°C | Carpoglyphus lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42800 | - |
x * 42800, SDS-PAGE | Carpoglyphus lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geraniol + NAD+ | Carpoglyphus lactis | the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone | geranial + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carpoglyphus lactis | B2NI94 | astigmatid mite | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1879fold to homogeneity by ammonium sulfate fractionation, followed by hydrophobic interactin and adsorption chromatography, anion exchange chromatography, and gel filtration in seven purification steps, overview | Carpoglyphus lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31 | - |
purified enzyme | Carpoglyphus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| E,E-farnesol + NAD+ | - |
Carpoglyphus lactis | ? | - |
? | |
| geraniol + NAD+ | the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone | Carpoglyphus lactis | geranial + NADH + H+ | - |
? | |
| geraniol + NAD+ | stereospecific reaction, no activity with nerol | Carpoglyphus lactis | geranial + NADH + H+ | - |
? | |
| additional information | substrate specificity, poor activity with citronellol, 3-methyl-2-buten-1-ol, 1-octanol, 1-butanol, 2-buten-1-ol, and ethanol, overview | Carpoglyphus lactis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42800, SDS-PAGE | Carpoglyphus lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GeDH | - |
Carpoglyphus lactis |
| More | the enzyme is a member of the medium-chain dehydrogenase/reductase family | Carpoglyphus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Carpoglyphus lactis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
60 | temprature profile, overview | Carpoglyphus lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Carpoglyphus lactis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | specific for, no activity with NADP+ | Carpoglyphus lactis | |
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