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Literature summary for 1.1.1.179 extracted from

  • Mihasan, M.; Stefan, M.; Hritcu, L.; Artenie, V.; Brandsch, R.
    Evidence of a plasmid-encoded oxidative xylose-catabolic pathway in Arthrobacter nicotinovorans pAO1 (2013), Res. Microbiol., 164, 22-30.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
NADP+ 28┬░C, pH 7.8 Paenarthrobacter nicotinovorans
5.03
-
NAD+ 28┬░C, pH 7.8 Paenarthrobacter nicotinovorans
15.2
-
D-xylose 28┬░C, pH 7.8, with NADP+ as cofactor Paenarthrobacter nicotinovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 2 mol Zn2+ per monomer Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
4 * 47000, SDS-PAGE Paenarthrobacter nicotinovorans

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NAD+ very low activity compared to NADP+ Paenarthrobacter nicotinovorans D-xylono-1,5-lactone + NADH + H+
-
?
D-xylose + NADP+
-
Paenarthrobacter nicotinovorans D-xylono-1,5-lactone + NADPH + H+
-
?
additional information at concentrations of up to 50 mM of L-xylose, D-ribose, D-arabinose, L-arabinose, D-glucose, D-galactose or D-fructose, the enzyme produces little NADPH and the specific activity toward these substrates are below 5% of the activity recorded with D-xylose Paenarthrobacter nicotinovorans ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 47000, SDS-PAGE Paenarthrobacter nicotinovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
NAD+ 28┬░C, pH 7.8 Paenarthrobacter nicotinovorans
10.5
-
NADP+ 28┬░C, pH 7.8 Paenarthrobacter nicotinovorans
14.9
-
D-xylose 28┬░C, pH 7.8, with NADP+ as cofactor Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
NAD+ very low activity compared to NADP+ Paenarthrobacter nicotinovorans
NADP+ strong preference for NADP+ Paenarthrobacter nicotinovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.298
-
NAD+ 28┬░C, pH 7.8 Paenarthrobacter nicotinovorans
0.98
-
D-xylose 28┬░C, pH 7.8, with NADP+ as cofactor Paenarthrobacter nicotinovorans
32
-
NADP+ 28┬░C, pH 7.8 Paenarthrobacter nicotinovorans