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Literature summary for 1.1.1.179 extracted from

  • Johnsen, U.; Dambeck, M.; Zaiss, H.; Fuhrer, T.; Soppa, J.; Sauer, U.; Schoenheit, P.
    D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii (2009), J. Biol. Chem., 284, 27290-27303.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information induced by D-xylose Haloferax volcanii

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain Haloferax volcanii
expression in Escherichia coli Haloferax volcanii

Protein Variants

Protein Variants Comment Organism
additional information in-frame deletion mutant can not grow on xylose but grows unaffected on glucose as the wild-type Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
NADP+
-
Haloferax volcanii
0.36
-
NAD+
-
Haloferax volcanii
0.75
-
NADP+ pH 8.3, 42┬░C Haloferax volcanii
4.7
-
D-xylose
-
Haloferax volcanii
8 9 D-xylose pH 8.3, 42┬░C Haloferax volcanii
198
-
D-glucose
-
Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5550
-
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis Haloferax volcanii
42300
-
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis Haloferax volcanii
165000
-
native enzyme Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADP+ Haloferax volcanii initial step in xylose degradation D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ Haloferax volcanii DS2 initial step in xylose degradation D-xylono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GP29 DS70 strain H26
-
Haloferax volcanii D4GP30
-
-
Haloferax volcanii DS2 D4GP30
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA column and by gel filtration Haloferax volcanii

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:xylose-grown cell
-
Haloferax volcanii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
7.8
-
with D-glucose as substrate Haloferax volcanii
10
-
pH 8.3, 42┬░C Haloferax volcanii
24.1
-
with D-xylose as substrate Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NAD(P)+
-
Haloferax volcanii D-gluconolactone + NAD(P)H
-
?
D-glucose + NADP+ 20% of the activity with D-xylose Haloferax volcanii D-glucono-1,5-lactone + NADPH + H+
-
?
D-glucose + NADP+ 20% of the activity with D-xylose Haloferax volcanii DS2 D-glucono-1,5-lactone + NADPH + H+
-
?
D-xylose + NAD(P)+ is highly specific for D-xylose Haloferax volcanii D-xylonolactone + NAD(P)H
-
?
D-xylose + NAD+
-
Haloferax volcanii D-xylono-1,5-lactone + NADH + H+
-
r
D-xylose + NADP+
-
Haloferax volcanii D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ initial step in xylose degradation Haloferax volcanii D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+
-
Haloferax volcanii DS2 D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ initial step in xylose degradation Haloferax volcanii DS2 D-xylono-1,5-lactone + NADPH + H+
-
?
additional information no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose Haloferax volcanii ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis Haloferax volcanii

Synonyms

Synonyms Comment Organism
D-xylose dehydrogenase
-
Haloferax volcanii
XDH
-
Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Haloferax volcanii
NADP+ no activity with NAD+ Haloferax volcanii