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Literature summary for 1.1.1.179 extracted from

  • Berghaell, S.; Hilditch, S.; Penttilae, M.; Richard, P.
    Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+):D-xylose dehydrogenase (2007), FEMS Microbiol. Lett., 277, 249-253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene xyd1, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, recombinant enzyme Trichoderma reesei
5.7
-
D-glucose pH 8.0, 30┬░C, recombinant enzyme Trichoderma reesei
24
-
NADP+ pH 8.1, 30┬░C, recombinant enzyme Trichoderma reesei
43
-
D-xylose pH 8.1, 30┬░C, recombinant enzyme Trichoderma reesei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42720
-
x * 42720, sequence calculation Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADP+ Trichoderma reesei best substrate D-xylono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei A8BT09 i.e. Trichoderma reesei, strain VTT-D-80133, gene xyd1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Saccharomyces cerevisiae by nickel affinity chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
substrate specificity Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NADP+
-
Trichoderma reesei D-galactono-1,5-lactone + NADPH
-
?
D-glucose + NADP+
-
Trichoderma reesei D-glucono-1,5-lactone + NADPH + H+
-
?
D-ribose + NADP+ low activity Trichoderma reesei ? + NADPH
-
?
D-xylose + NADP+ best substrate Trichoderma reesei D-xylono-1,5-lactone + NADPH + H+
-
?
L-arabinose + NADP+
-
Trichoderma reesei L-arabinono-1,4-lactone + NADPH
-
?
additional information no or poor activity with D-arabinose, D-mannose, L-rhamnose, D-lyxose, D-fructose, D-glyceraldehyde, and DL-glyceraldehyde Trichoderma reesei ?
-
?

Subunits

Subunits Comment Organism
? x * 42720, sequence calculation Trichoderma reesei

Synonyms

Synonyms Comment Organism
NADP+-dependent D-xylose dehydrogenase
-
Trichoderma reesei
NADP+:D-xylose dehydrogenase
-
Trichoderma reesei

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.7
-
D-glucose pH 8.0, 30┬░C, recombinant enzyme Trichoderma reesei
21.8
-
D-xylose pH 8.1, 30┬░C, recombinant enzyme Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
assay at Trichoderma reesei

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on, specific for Trichoderma reesei