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Literature summary for 1.1.1.179 extracted from

  • Carbone, V.; Sumii, R.; Ishikura, S.; Asada, Y.; Hara, A.; El-Kabbani, O.
    Structure of monkey dimeric dihydrodiol dehydrogenase in complex with isoascorbic acid (2008), Acta Crystallogr. Sect. D, 64, 532-542.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3) Macaca fascicularis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are obtained at 19.9Ā°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric D-xylose dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution Macaca fascicularis

Protein Variants

Protein Variants Comment Organism
A36D site-directed mutagenesis Macaca fascicularis
D280A by site-directed mutagenesis Macaca fascicularis
F154A by site-directed mutagenesis Macaca fascicularis
F279A by site-directed mutagenesis Macaca fascicularis
H76Q by site-directed mutagenesis Macaca fascicularis
H79Q by site-directed mutagenesis Macaca fascicularis
K97M by site-directed mutagenesis Macaca fascicularis
K97R by site-directed mutagenesis Macaca fascicularis
R37A site-directed mutagenesis Macaca fascicularis
R37D site-directed mutagenesis Macaca fascicularis
R41A by site-directed mutagenesis Macaca fascicularis
R41D by site-directed mutagenesis Macaca fascicularis
W125Y by site-directed mutagenesis Macaca fascicularis
W254A by site-directed mutagenesis Macaca fascicularis
W254Y by site-directed mutagenesis Macaca fascicularis

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyacetophenone
-
Macaca fascicularis
isoascorbic acid competitive inhibitor Macaca fascicularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
D-xylose oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
2.9
-
D-xylose oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
3.4
-
D-xylose oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
4.4
-
D-xylose oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
8 9 D-xylose oxidation reaction of mutant F279A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
11
-
D-xylose oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
14
-
D-xylose oxidation reaction of mutant W254Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
15
-
D-xylose oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
36
-
D-xylose oxidation reaction of mutant F154A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
41
-
D-xylose oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
65
-
D-xylose oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
67
-
D-xylose oxidation reaction of mutant W125Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis
90
-
D-xylose oxidation reaction of mutant W254A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose Macaca fascicularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADP+ Macaca fascicularis enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase D-xylono-1,5-lactone + NADPH + H+
-
?
additional information Macaca fascicularis D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation ?
-
?

Organism

Organism UniProt Comment Textmining
Macaca fascicularis Q9TQS6
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzymes are purified from the 12000g supernatants of the homogenates of the cells Macaca fascicularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADP+ enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase Macaca fascicularis D-xylono-1,5-lactone + NADPH + H+
-
?
additional information D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation Macaca fascicularis ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Macaca fascicularis

Synonyms

Synonyms Comment Organism
NADP+-dependent D-xylose dehydrogenase
-
Macaca fascicularis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
D-xylose oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
0.45
-
D-xylose oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
0.5
-
D-xylose oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
0.9
-
D-xylose oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
1
-
D-xylose oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
1.2
-
D-xylose oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
1.7
-
D-xylose oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis
2.6
-
D-xylose oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose Macaca fascicularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Macaca fascicularis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Macaca fascicularis