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Literature summary for 1.1.1.179 extracted from

  • Johnsen, U.; Schonheit, P.
    Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui (2004), J. Bacteriol., 186, 6198-6207.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Haloarcula marismortui

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
D-xylose pH 8.3, 37┬░C Haloarcula marismortui
2.3
-
D-ribose pH 8.3, 37┬░C Haloarcula marismortui
64
-
D-glucose pH 8.3, 37┬░C Haloarcula marismortui

Metals/Ions

Metals/Ions Comment Organism Structure
KCl maximal activity at 1.5 M Haloarcula marismortui
MgCl2 maximal activity at 100 M Haloarcula marismortui
NaCl maximal activity at 1.5 M Haloarcula marismortui

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39900
-
4 * 39900, SDS-PAGE Haloarcula marismortui
175000
-
gel filtration Haloarcula marismortui

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADP+ Haloarcula marismortui initial step in xylose degradation D-xylonolactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Haloarcula marismortui Q5UY95
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloarcula marismortui

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:xylose-grown cell
-
Haloarcula marismortui
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
100
-
pH 8.8, 37┬░C Haloarcula marismortui

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+ reaction is catalyzed with 70fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP* Haloarcula marismortui D-gluconolactone + NADPH + H+
-
?
D-ribose + NADP+ reaction is catalyzed with 1.8fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP* Haloarcula marismortui D-ribonolactone + NADPH + H+
-
?
D-xylose + NAD+ Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui D-xylonolactone + NADH + H+
-
?
D-xylose + NADP+ initial step in xylose degradation Haloarcula marismortui D-xylonolactone + NADPH + H+
-
?
D-xylose + NADP+ Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui D-xylonolactone + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 39900, SDS-PAGE Haloarcula marismortui

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Haloarcula marismortui
50
-
-
Haloarcula marismortui

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
25 50 activity increases exponentially with temperature between 25┬░C and 45┬░C. 50┬░C: optimum Haloarcula marismortui

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
273
-
D-glucose pH 8.3, 37┬░C Haloarcula marismortui
356
-
D-xylose pH 8.3, 37┬░C Haloarcula marismortui
360
-
D-ribose pH 8.3, 37┬░C Haloarcula marismortui

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Haloarcula marismortui

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 50% of maximal activity Haloarcula marismortui

Cofactor

Cofactor Comment Organism Structure
NAD+ Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui
NADP+ preferred electron acceptor. Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.043
-
D-glucose pH 8.3, 37┬░C Haloarcula marismortui
1.57
-
D-ribose pH 8.3, 37┬░C Haloarcula marismortui
2.97
-
D-xylose pH 8.3, 37┬░C Haloarcula marismortui