Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.179 extracted from

  • Aoki, S.; Ishikura, S.; Asada, Y.; Usami, N.; Hara, A.
    Identity of dimeric dihydrodiol dehydrogenase as NADP+-dependent D-xylose dehydrogenase in pig liver (2001), Chem. Biol. Interact., 130-132, 775-784.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
KCl increase in activity about 2fold Sus scrofa
MgCl2 increase in activity about 4fold Sus scrofa
additional information
-
Macaca fuscata
additional information higher salt concentration elevates the activity Sus scrofa

Protein Variants

Protein Variants Comment Organism
H79Q large increase in Km for coenzyme and substrates Macaca fuscata
Y180F
-
Macaca fuscata

Inhibitors

Inhibitors Comment Organism Structure
high salt concentration recombinant H79Q mutant Macaca fuscata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
NADP+ + 20 mM MgCl2 Sus scrofa
1.3
-
D-xylose 25 mM Tris + 20 mM MgCl2 Sus scrofa
6.4
-
D-xylose
-
Macaca fuscata
6.5
-
D-xylose
-
Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADP+ Homo sapiens
-
D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ Sus scrofa
-
D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ Oryctolagus cuniculus
-
D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ Canis lupus familiaris
-
D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+ Macaca fuscata
-
D-xylono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
-
-
Macaca fuscata
-
-
-
Oryctolagus cuniculus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa
-
Oryctolagus cuniculus
-
Canis lupus familiaris
-
Macaca fuscata

Reaction

Reaction Comment Organism Reaction ID
D-xylose + NADP+ = D-xylono-1,5-lactone + NADPH + H+ ordered bi-bi mechanism Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
intestine exclusively Homo sapiens
-
kidney exclusively Macaca fuscata
-
lens
-
Sus scrofa
-
lens
-
Oryctolagus cuniculus
-
lens
-
Canis lupus familiaris
-
liver
-
Sus scrofa
-
liver
-
Canis lupus familiaris
-
additional information enzyme activity is found in almost all tissues Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
7.6
-
-
Sus scrofa
9
-
-
Macaca fuscata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADP+
-
Homo sapiens D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+
-
Sus scrofa D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+
-
Oryctolagus cuniculus D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+
-
Canis lupus familiaris D-xylono-1,5-lactone + NADPH + H+
-
?
D-xylose + NADP+
-
Macaca fuscata D-xylono-1,5-lactone + NADPH + H+
-
?
additional information
-
Sus scrofa ?
-
?
additional information catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20 Homo sapiens ?
-
?
additional information catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20 Oryctolagus cuniculus ?
-
?
additional information catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20 Canis lupus familiaris ?
-
?
additional information catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20 Macaca fuscata ?
-
?
trans-benzene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Sus scrofa ?
-
?
trans-benzene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Oryctolagus cuniculus ?
-
?
trans-benzene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Canis lupus familiaris ?
-
?
trans-benzene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Macaca fuscata ?
-
?
trans-naphthalene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Sus scrofa ?
-
?
trans-naphthalene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Oryctolagus cuniculus ?
-
?
trans-naphthalene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Canis lupus familiaris ?
-
?
trans-naphthalene dihydrodiol + NADP+ dihydrodiol dehydrogenase activity, probably identical with EC 1.3.1.20 Macaca fuscata ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sus scrofa
7.5
-
assay at Oryctolagus cuniculus
7.5
-
assay at Canis lupus familiaris
7.5
-
assay at Macaca fuscata
10
-
dihydrodiol dehydrogenase activity assay at Sus scrofa
10
-
dihydrodiol dehydrogenase activity assay at Oryctolagus cuniculus
10
-
dihydrodiol dehydrogenase activity assay at Canis lupus familiaris
10
-
dihydrodiol dehydrogenase activity assay at Macaca fuscata

Cofactor

Cofactor Comment Organism Structure
additional information
-
Sus scrofa
additional information Tyr71, His79, Lys97 involved in coenzyme binding Macaca fuscata
NADP+
-
Homo sapiens
NADP+
-
Sus scrofa
NADP+
-
Oryctolagus cuniculus
NADP+
-
Canis lupus familiaris
NADP+ Tyr71, His79, Lys97 involved in coenzyme binding Macaca fuscata