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Literature summary for 1.1.1.179 extracted from

  • Asada, Y.; Aoki, S.; Ishikura, S.; Usami, N.; Hara, A.
    Roles of His-79 and Tyr-180 of D-xylose/dihydrodiol dehydrogenase in catalytic function (2000), Biochem. Biophys. Res. Commun., 278, 333-337.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information
-
Macaca fuscata

Cloned(Commentary)

Cloned (Comment) Organism
cDNA subcloned in expression vector pRset, overexpression in Escherichia coli Macaca fuscata

Protein Variants

Protein Variants Comment Organism
H79Q increase in Km more than 25fold, increase in Kd more than 7fold, with high ionic strength Km increased by 190fold and inhibited the enzyme Macaca fuscata
Y180F almost inactive, high affinity for NADP(H) Macaca fuscata

Inhibitors

Inhibitors Comment Organism Structure
high salt concentration recombinant H79Q mutant Macaca fuscata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information phosphate buffer increases the Km-value, especially for mutant H79Q, compared with Tris buffer Macaca fuscata
0.0006
-
NADPH camphorquinone Macaca fuscata
0.0006
-
NADP+ D-xylose Macaca fuscata
0.014
-
NADP+ D-xylose, mutant H79Q Macaca fuscata
0.028
-
NADPH camphorquinone, mutant H79Q Macaca fuscata
0.1
-
Camphorquinone
-
Macaca fuscata
0.51
-
Camphorquinone mutant H79Q Macaca fuscata
4.4
-
D-xylose
-
Macaca fuscata
67
-
D-xylose mutant H79Q Macaca fuscata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Macaca fuscata
70000
-
about, gel filtration Macaca fuscata

Organism

Organism UniProt Comment Textmining
Macaca fuscata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme and mutants Y180F, H79Q Macaca fuscata

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Macaca fuscata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
5.3 5.5 wild-type and mutant H79Q Macaca fuscata
18
-
substrate camphorquinone, mutant H79Q Macaca fuscata
33
-
substrate camphorquinone Macaca fuscata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
camphorquinone + NADPH
-
Macaca fuscata (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
?
D-xylose + NADP+
-
Macaca fuscata D-xylono-1,5-lactone + NADPH + H+
-
?
additional information catalyzing oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols, probably identical with dimeric dihydrodiol dehydrogenase EC 1.3.1.20 Macaca fuscata ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Macaca fuscata

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Macaca fuscata