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Literature summary for 1.1.1.176 extracted from

  • Fossati, E.; Polentini, F.; Carrea, G.; Riva, S.
    Exploitation of the alcohol dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid (2006), Biotechnol. Bioeng., 93, 1216-1220.
    View publication on PubMed
Search for more literature for 1.1.1.176

Inhibitors

Inhibitors Comment Organism Structure
acetone at 25% v/v and above Clostridium sp.
additional information no substrate inhibition at high concentration of cholic acid Clostridium sp.

Organism

Organism UniProt Comment Textmining
Clostridium sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation enzyme in 50% glycerol-containing solution Clostridium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay method development using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, optimization, overview Clostridium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholic acid + NADP+ the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview Clostridium sp. 12-ketochenodeoxycholic acid + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
12alpha-HSDH
-
 Clostridium sp.
NADP-dependent 12alpha-hydroxysteroid dehydrogenase
-
 Clostridium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Clostridium sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Clostridium sp.