General Stability | Organism |
---|---|
glycerol, 50% v/v, stabilization | Clostridium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Clostridium sp. | |
Cu2+ | - |
Clostridium sp. | |
Hg2+ | - |
Clostridium sp. | |
p-chloromercuribenzoate | - |
Clostridium sp. | |
pyridoxal 5'-phosphate | protection by NADP+ or NADPH | Clostridium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
12-oxochenodeoxycholic acid | - |
Clostridium sp. | |
0.0085 | - |
NADPH | - |
Clostridium sp. | |
0.035 | - |
NADP+ | - |
Clostridium sp. | |
0.045 | - |
deoxycholic acid | - |
Clostridium sp. | |
0.072 | - |
cholic acid | - |
Clostridium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
4 * 26000, SDS-PAGE | Clostridium sp. |
26000 | - |
4 * 26000, no disulfide bonds, SDS-PAGE, electron microscopy | Clostridium sp. |
98000 | 106000 | native PAGE, analytical ultracentrifugation | Clostridium sp. |
102000 | 108000 | native PAGE, analytical ultracentrifugation, FPLC gel filtration | Clostridium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sp. | - |
group P, strain 48-50 | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium sp. |
Storage Stability | Organism |
---|---|
-30°C, 6 months, 50% glycerol, no loss of activity | Clostridium sp. |
-30°C, concentrated form, at least 6 months | Clostridium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-oxochenodeoxycholic acid + NADP+ | - |
Clostridium sp. | (3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid + NADPH + H+ | - |
? | |
cholic acid + NAD(P)+ | hardly accepts N6-modified NADP+ derivative | Clostridium sp. | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
cholic acid + NAD(P)+ | hardly accepts N6-modified NADP+ derivative | Clostridium sp. | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
cholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid | Clostridium sp. | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
cholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid | Clostridium sp. | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
deoxycholic acid + NAD(P)+ | i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid | Clostridium sp. | 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 26000, SDS-PAGE | Clostridium sp. |
tetramer | 4 * 26000, no disulfide bonds, SDS-PAGE, electron microscopy | Clostridium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
optimum above 55°C, not measurable because enzyme is inactivated above 55°C | Clostridium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
half-life: 4 days, increases to 10 days by addition of NADP+ | Clostridium sp. |
30 | - |
half-life: 2 days, increases to 5 days by addition of NADP+ | Clostridium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
reduction of 12-oxochenodeoxycholic acid optimal below pH 5.0, not measurable due to insolubility of substrate | Clostridium sp. |
8.5 | 9.5 | oxidation of cholic acid | Clostridium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridium sp. | |
NADP+ | - |
Clostridium sp. | |
NADPH | - |
Clostridium sp. |