Application | Comment | Organism |
---|---|---|
synthesis | expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0 | Caulobacter vibrioides |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Caulobacter vibrioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
D-xylose | 25°C, pH 9.0 | Caulobacter vibrioides | |
0.24 | - |
D-xylose | 25°C, pH 7.0 | Caulobacter vibrioides | |
0.26 | - |
NAD+ | 25°C, pH 6.8 | Caulobacter vibrioides | |
0.31 | - |
NAD+ | 25°C, pH 7.0 | Caulobacter vibrioides | |
0.4 | - |
D-xylose | 25°C, pH 6.8 | Caulobacter vibrioides | |
0.46 | - |
NAD+ | 25°C, pH 9.0 | Caulobacter vibrioides | |
40 | - |
L-arabinose | 25°C, pH 9.0 | Caulobacter vibrioides | |
100 | - |
L-arabinose | 25°C, pH 7.0 | Caulobacter vibrioides | |
180 | - |
L-arabinose | 25°C, pH 6.8 | Caulobacter vibrioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | Caulobacter vibrioides | - |
D-xylonolactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | B8H1Z0 | putative | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | - |
Caulobacter vibrioides | D-xylonolactone + NADH + H+ | - |
? | |
L-arabinose + NAD+ | - |
Caulobacter vibrioides | L-arabinono-1,4-lactone + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.3 | - |
L-arabinose | 25°C, pH 6.8 | Caulobacter vibrioides | |
22.7 | - |
D-xylose | 25°C, pH 9.0 | Caulobacter vibrioides | |
24.2 | - |
L-arabinose | 25°C, pH 9.0 | Caulobacter vibrioides | |
25.8 | - |
NAD+ | 25°C, pH 6.8 | Caulobacter vibrioides | |
26.2 | - |
D-xylose | 25°C, pH 6.8 | Caulobacter vibrioides | |
29.2 | - |
L-arabinose | 25°C, pH 7.0 | Caulobacter vibrioides | |
30.3 | - |
D-xylose | 25°C, pH 7.0 | Caulobacter vibrioides | |
31 | - |
NAD+ | 25°C, pH 9.0 | Caulobacter vibrioides | |
35.7 | - |
NAD+ | 25°C, pH 7.0 | Caulobacter vibrioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Caulobacter vibrioides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-arabinose | 25°C, pH 6.8 | Caulobacter vibrioides | |
0.28 | - |
L-arabinose | 25°C, pH 7.0 | Caulobacter vibrioides | |
0.6 | - |
L-arabinose | 25°C, pH 9.0 | Caulobacter vibrioides | |
66.7 | - |
D-xylose | 25°C, pH 6.8 | Caulobacter vibrioides | |
66.7 | - |
NAD+ | 25°C, pH 9.0 | Caulobacter vibrioides | |
101.7 | - |
NAD+ | 25°C, pH 6.8 | Caulobacter vibrioides | |
115 | - |
NAD+ | 25°C, pH 7.0 | Caulobacter vibrioides | |
125 | - |
D-xylose | 25°C, pH 7.0 | Caulobacter vibrioides | |
280 | - |
D-xylose | 25°C, pH 9.0 | Caulobacter vibrioides |