Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5alpha-cholest-7-en-3-one | 2-9.3% inhibition between 16 and 130 nmol | Rattus sp. | |
5alpha-Cholest-7-en-3beta-ol | 15.5-65% inhibition between 30 and 260 nmol | Rattus sp. | |
Fe2+ | less than 25% inhibition at 1 mM | Rattus sp. | |
Zn2+ | marked inhibition between 0.1 and 1 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid | - |
Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy-4alpha-methyl-5alpha-cholest-7-ene-4beta-carboxylate + NAD+ | Rattus sp. | - |
4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH | - |
? | |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid + NAD+ | Rattus sp. | no epimerization of 4beta-isomer into 4alpha-isomer | 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phospholipoprotein | presence of phosphatidylcholine and phosphatidylethanolamine, no loss of activity after removal of phospholipids | Rattus sp. |
Purification (Comment) | Organism |
---|---|
near homogeneity, chromatography techniques, sodium deoxycholate solubilization | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
- |
Rattus sp. |
Storage Stability | Organism |
---|---|
4°C, 10 mM phosphate buffer, pH 7.4, no loss of activity within 2 weeks | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy-4alpha-methyl-5alpha-cholest-7-ene-4beta-carboxylate + NAD+ | - |
Rattus sp. | 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH | - |
? | |
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid + NAD+ | no epimerization of 4beta-isomer into 4alpha-isomer | Rattus sp. | 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
4alpha-carboxylic acid decarboxylase | - |
Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Rattus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
acidic pH results in a very low decarboxylation rate | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can not be replaced by NADP+ | Rattus sp. |