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Literature summary for 1.1.1.17 extracted from

  • Zeidler, S.; Hubloher, J.; Koenig, P.; Ngu, N.; Scholz, A.; Averhoff, B.; Mueller, V.
    Salt induction and activation of MtlD, the key enzyme in the synthesis of the compatible solute mannitol in Acinetobacter baumannii (2018), MicrobiologyOpen, 7, e00614 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.17

Activating Compound

Activating Compound Comment Organism Structure
Na2SO4 at 1 M, 35% of maximum activity obtained with NaCl Acinetobacter baumannii
NaNO3 at 0.6 M, 25% of maximum activity obtained with NaCl Acinetobacter baumannii
sodium gluconate at 1.5 M, 135% of maximum activity obtained with NaCl Acinetobacter baumannii
sodium glutamate highest stimulations up to 41 U/mg at 0.8-1.2 M Acinetobacter baumannii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter baumannii

Inhibitors

Inhibitors Comment Organism Structure
KCl at 1.5 M, 52% of maximum activity obtained with NaCl Acinetobacter baumannii
NaCl activity is strictly salt dependent, with highest stimulation at 600 mM NaCl and 24% of maximum activity at 1.5 M Acinetobacter baumannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
55
-
 D-fructose 6-phosphate pH 7.0, 37°C Acinetobacter baumannii

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activity is strictly salt dependent, with highest stimulation at 600 mM NaCl Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 gel filtration Acinetobacter baumannii

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii D0C7J2
-
-
Acinetobacter baumannii ATCC 19606 D0C7J2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 presence of 600 mM NaCl, pH 7, 37°C Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + NADH + H+
-
 Acinetobacter baumannii D-mannitol 1-phosphate + NAD+
-
 ?
D-fructose 6-phosphate + NADH + H+
-
 Acinetobacter baumannii ATCC 19606 D-mannitol 1-phosphate + NAD+
-
 ?
D-fructose 6-phosphate + NADPH + H+
-
 Acinetobacter baumannii D-mannitol 1-phosphate + NADP+
-
 ?
D-fructose 6-phosphate + NADPH + H+
-
 Acinetobacter baumannii ATCC 19606 D-mannitol 1-phosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 82000, SDS-PAGE recombinant His-tagged protein Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
HAD hydrolase, family IA, variant 3
-
 Acinetobacter baumannii
HMPREF0010_00722
-
 Acinetobacter baumannii
MtlD
-
 Acinetobacter baumannii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Acinetobacter baumannii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 40% of the activity at 37°C Acinetobacter baumannii
45
-
 40% of the activity at 37°C Acinetobacter baumannii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Acinetobacter baumannii

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 12% of maximum activity Acinetobacter baumannii
10
-
 12% of maximum activity Acinetobacter baumannii

Cofactor

Cofactor Comment Organism Structure
NADH 5fold decrease in activity compared with NADPH Acinetobacter baumannii
NADPH NADPH is preferred over NADH Acinetobacter baumannii

Expression

Organism Comment Expression
Acinetobacter baumannii the presence of glycine betaine or choline repress promoter activation down
Acinetobacter baumannii expression of MtlD is strongly dependent on high osmolarity, not discriminating between different salts or sugars up

General Information

General Information Comment Organism
physiological function after deletion of the mtlD gene, cells no longer accumulate mannitol and growth is completely impaired at high salt concentrations Acinetobacter baumannii