Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21(DE3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, pH-dependence of kinetics with different substrates, overview | Escherichia coli | |
0.004 | - |
beta-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.018 | - |
thio-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.036 | - |
alpha-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.075 | - |
3'-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
0.12 | - |
2-oxopantoate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
3.8 | - |
3-methyl-2-oxo-n-valerate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
8.7 | - |
2-oxoisovalerate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxopantoate + NADPH | Escherichia coli | part of the pantothenate biosynthesis | (R)-pantoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene panE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate + NADPH | low activity | Escherichia coli | 2-hydroxyvalerate + NADP+ | - |
r | |
2-oxopantoate + 3'-NADPH | - |
Escherichia coli | (R)-pantoate + 3'-NADP+ | - |
r | |
2-oxopantoate + alpha-NADPH | - |
Escherichia coli | (R)-pantoate + alpha-NADP+ | - |
r | |
2-oxopantoate + beta-NADPH | highly specific for | Escherichia coli | (R)-pantoate + beta-NADP+ | - |
r | |
2-oxopantoate + NADPH | part of the pantothenate biosynthesis | Escherichia coli | (R)-pantoate + NADP+ | - |
? | |
2-oxopantoate + thio-NADPH | - |
Escherichia coli | (R)-pantoate + thio-NADP+ | - |
r | |
3-methyl-2-oxo-n-valerate + NADPH | low activity | Escherichia coli | 2-hydroxy-3-methyl-n-valerate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ketopantoate reductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of kinetics with different substrates, modeling, overview | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3'-NADPH | - |
Escherichia coli | |
alpha-NADPH | - |
Escherichia coli | |
beta-NADPH | preference for beta-NADPH as cofactor | Escherichia coli | |
thio-NADPH | - |
Escherichia coli |