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Literature summary for 1.1.1.166 extracted from

  • Whiting, G.C.; Coggins, R.A.
    A new nicotinamide-adenine dinucleotide-dependent hydroaromatic dehydrogenase of Lactobacillus plantarum and its role in formation of (-)t-3,t-4-dihydrocyclohexane-c-1carboxylate (1974), Biochem. J., 141, 35-42.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.166

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 NAD+ with (-)-shikimate Lactiplantibacillus plantarum
0.34
-
 NAD+ with (+-)t-3,t-4-dihydroxycyclohexanecarboxylate and (-)-dihydroshikimate Lactiplantibacillus plantarum
0.36
-
 NAD+ with (-)t-3,t-4-dihydroxycyclohexanecarboxylate Lactiplantibacillus plantarum
0.45
-
 NAD+ with (-)-quinate Lactiplantibacillus plantarum
0.52
-
 (-)-Dihydroshikimate
-
 Lactiplantibacillus plantarum
0.74
-
 (-)t-3,t-4-dihydroxycyclohexanecarboxylate
-
 Lactiplantibacillus plantarum
0.75
-
 (-)-shikimate
-
 Lactiplantibacillus plantarum
0.85
-
 (-)-quinate
-
 Lactiplantibacillus plantarum
1.55
-
 (+-)t-3,t-4-dihydroxycyclohexanecarboxylate
-
 Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1S,3R,4S)-3,4-dihydroxycyclohexane-1-carboxylate + NAD+ Lactiplantibacillus plantarum
-
 (1S,4S)-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Lactiplantibacillus plantarum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Lactiplantibacillus plantarum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.9
-
-
 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)t-3,t-4-dihydroxycyclohexanecarboxylate + NAD+
-
 Lactiplantibacillus plantarum 4-hydroxy-3-oxocyclohexanecarboxylate + NADH + H+
-
 r
(+-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate + NAD+
-
 Lactiplantibacillus plantarum (+-)t-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
 ?
(-)-dihydroshikimate + NAD+
-
 Lactiplantibacillus plantarum ? + NADH
-
 ?
(-)-quinate + NAD+
-
 Lactiplantibacillus plantarum 3-dehydroquinate + NADH + H+
-
 r
(-)-shikimate + NAD+ more rapidly oxidized than other acids Lactiplantibacillus plantarum (-)-3-dehydroshikimate + NADH + H+
-
 r
(-)t-3,t-4-dihydroxycyclohexanecarboxylate + NAD+
-
 Lactiplantibacillus plantarum 4-hydroxy-3-oxocyclohexanecarboxylate + NADH + H+
-
 r
(1S,3R,4S)-3,4-dihydroxycyclohexane-1-carboxylate + NAD+
-
 Lactiplantibacillus plantarum (1S,4S)-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 reductive direction Lactiplantibacillus plantarum
10 10.1 oxidative direction Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 active within, reductive direction Lactiplantibacillus plantarum
6.9 11.4 active within, oxidative direction Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
NAD+ can not be replaced by NADP+ Lactiplantibacillus plantarum