General Stability | Organism |
---|---|
highly unstable, more than 50% loss of activity within 24 h | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rifampicin | inhibits enzyme induction in hexadecanol-grown cells, not in cells grown on ethanol, palmitate or hexadecane | Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
hexadecanol | growth on ethanol, NAD+ | Acinetobacter sp. | |
0.0073 | - |
hexadecanol | growth on palmitic acid, NAD+ | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexadecanal + NADH | Acinetobacter sp. | inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane | hexadecanol + NAD+ | - |
? | |
hexadecanal + NADH | Acinetobacter sp. HO1-N | inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane | hexadecanol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. HO1-N | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Acinetobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl 10 mM, pH 7.8, precipitation at 50% ammonium sulfate saturation, dissolved in 15fold reduced volume | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexadecanal + NADH | inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane | Acinetobacter sp. | hexadecanol + NAD+ | - |
? | |
hexadecanal + NADH | inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane | Acinetobacter sp. HO1-N | hexadecanol + NAD+ | - |
? | |
additional information | enzyme is not visible as enzymatically active protein in zymograms | Acinetobacter sp. | ? | - |
? | |
additional information | enzyme is not visible as enzymatically active protein in zymograms | Acinetobacter sp. HO1-N | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preference for | Acinetobacter sp. |