Any feedback?
Literature summary for 1.1.1.159 extracted from
- Modifying the microenvironment of epoxy resin to improve the activity of immobilized 7alpha-hydroxysteroid dehydrogenases (2020), Appl. Biochem. Biotechnol., 193, 925-939 .
General Stability
| General Stability | Organism |
|---|---|
| the relative activity of immobilized enzyme on aminoepoxy resin increases by approximately 80%. After 6 cycles, the residual activities of the enzyme immobilized on the amino-epoxy resin and epoxy resin are 81.4% and 89.5%, respectively | Clostridium sardiniense |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| taurochenodeoxycholic acid + NADP+ | Clostridium sardiniense | - |
tauro-7-ketolithocholic acid + NADPH + H+ | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium sardiniense | G9FRD7 | - |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris-HCl (pH 9.0), 2 weeks, 24.9% loss of activity, immobilized enzyme | Clostridium sardiniense |
| 4°C, 50 mM Tris-HCl (pH 9.0), 5 weeks, 60.3% loss of activity, immobilized enzyme | Clostridium sardiniense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| taurochenodeoxycholic acid + NADP+ | - |
Clostridium sardiniense | tauro-7-ketolithocholic acid + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7alpha-HSDH | - |
Clostridium sardiniense |
| nicotinamide adenine dinucleotide phosphate-dependent 7alpha-hydroxysteroid dehydrogenase | - |
Clostridium sardiniense |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Clostridium sardiniense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 65 | the relative activity of the free enzyme drastically decreases at temperatures over 35°C, with minimal activity observed at 45°C. In contrast, the immobilized preparations are relatively stable at temperatures ranging from 15 to 35°C, with the relative activities of enzyme immobilized on the amino-epoxy resin and epoxy resin at 56.4% and 61.0%, respectively, after 2 h at 65°C | Clostridium sardiniense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Clostridium sardiniense |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
the optimum conditions for the immobilization of the enzyme on both amino-epoxy resin and epoxy resin are pH 9.0 and 20°C for 2 h | Clostridium sardiniense |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Clostridium sardiniense | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridium sardiniense | isoelectric focusing | - |
5.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
